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Invitrogen
IKAROS Polyclonal Antibody
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FIGURE: 1 / 7
IKAROS Antibody (PA5-47682) in ICC/IF
Immunofluorescence analysis of IKZF1 was performed using log phase REH and HL60 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with IKAROS Polyclonal Antibody (Product # PA5-47682) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Product # A11078), (1:2000 dilution), for ... View More
Product Details
PA5-47682
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant human lkaros Ser427-Ser519
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinat human (rh) ZIC-1, rhZNF-24, and rhZNF-206 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
DNA-binding protein IKAROS functions in the specification and the maturation of the T lymphocyte. It also interacts with a critical control element in the terminal deoxynucleotidyl transferase promoter as well as with the promoters for other genes expressed during early stages of B-cell and T-cell development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CLL-associated antigen KW-6; DNA-binding protein Ikaros; hIk-1; Ikaros family zinc finger protein 1; Ikaros zinc finger protein; zinc finger protein subfamily 1A 1; DNA-binding protein Ikaros; CLL-associated antigen KW-6; Ikaros family zinc finger protein 1; lymphoid transcription factor LyF-1; lymphocyte-restricted zinc finger DNA binding protein; Lymphoid transcription factor LyF-1; predicted protein of HQ0758; protein phosphatase 1, regulatory subunit 92; unnamed protein product; zinc finger protein, subfamily 1A, 1 (Ikaros); zinc finger transcription factor
Gene Aliases: CVID13; Hs.54452; IK1; IKAROS; IKZF1; LyF-1; LYF1; PPP1R92; PRO0758; ZNFN1A1
UniProt ID: (Human) Q13422
Entrez Gene ID: (Human) 10320
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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