|Tested species reactivity||Bovine, Human|
|Published species reactivity||Pig, Sheep, Bovine, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant human IL-1 alpha|
|Purification||Ammonium sulfate precipitation|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
P420A targets IL-1 alpha in WB applications and shows reactivity with Bovine, and Human samples.
The P420A immunogen is recombinant human IL-1 alpha.
P420A detects IL-1 alpha which has a predicted molecular weight of approximately 18 kDa.
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Potential role of proinflammatory cytokines in the pathogenetic mechanisms of vascular lesions in goats naturally infected with bluetongue virus serotype 1.
P420A was used in immunohistochemistry to study the role of bluetongue virus serotype-1-induced vasoactive cytokines in the pathogenesis of vascular lesions in goats naturally infected with the virus
|Sánchez-Cordón PJ,Pedrera M,Risalde MA,Molina V,Rodríguez-Sánchez B,Núñez A,Sánchez-Vizcaíno JM,Gómez-Villamandos JC||Transboundary and emerging diseases (60:252)||2013|
Distinct expression of interleukin-1¿, interleukin-1ß, and interleukin-1 receptor antagonist in testicular tissues and cells from human biopsies with normal and abnormal histology.
P420A was used in immunohistochemistry to investigate the level of interleukin-1??, interleukin-1??, and interleukin-1 receptor antagonist in human testicular normal and abnormal biopsies
|Abu Elheija M,Dyomin V,Ganaiem M,Lunenfeld E,Vardy NS,Huleihel M||Journal of interferon and cytokine research : the official journal of the International Society for Interferon and Cytokine Research (31:401)||2011|
Expression of genes associated with immunity in the endometrium of cattle with disparate postpartum uterine disease and fertility.
P420A was used in immunohistochemistry to investigate the changes of gene expression in cattle endometrium with postpartum bacterial infection
|Herath S,Lilly ST,Santos NR,Gilbert RO,Goetze L,Bryant CE,White JO,Cronin J,Sheldon IM||Reproductive biology and endocrinology : RB and E (7:null)||2009|
Changes in macrophages in spleen and lymph nodes during acute African swine fever: expression of cytokines.
P420A was used in immunohistochemistry to study the role of cytokine expression by macrophages in spleen and lymph nodes in the pathogenesis of African swine fever
|Salguero FJ,Ruiz-Villamor E,Bautista MJ,Sánchez-Cordón PJ,Carrasco L,Gómez-Villamandos JC||Veterinary immunology and immunopathology (90:11)||2002|
Macrophage- and neutrophil-dominant arthritis in human IL-1 alpha transgenic mice.
P420A was used in immunohistochemistry and immunoprecipitation to study the role of IL-1alpha in arthritis using transgenic mice expressiong human IL-1alpha
|Niki Y,Yamada H,Seki S,Kikuchi T,Takaishi H,Toyama Y,Fujikawa K,Tada N||The Journal of clinical investigation (107:1127)||2001|
Induction of IL-3 and granulocyte-macrophage colony-stimulating factor by substance P in bone marrow cells is partially mediated through the release of IL-1 and IL-6.
P420A was used in blocking/activating experiment to investigate the production of interleukin 3 and GM-CSF mediated by substance P in bone marrow mononuclear cells
|Rameshwar P,Ganea D,Gascón P||Journal of immunology (Baltimore, Md. : 1950) (152:4044)||1994|