|Tested species reactivity||Human, Mouse, Non-human primate|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant human IL-1 beta|
|Purification||Ammonium sulfate precipitation|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Neutralization (Neu)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
P420B targets IL-1 beta in ELISA, IHC, Neu, and WB applications and shows reactivity with Human, mouse, and Non-human primate samples.
The P420B immunogen is recombinant human IL-1 beta.
P420B detects IL-1 beta which has a predicted molecular weight of approximately 31 kDa.
This product is a Low Endotoxin formulation.
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Distinct expression of interleukin-1¿, interleukin-1ß, and interleukin-1 receptor antagonist in testicular tissues and cells from human biopsies with normal and abnormal histology.
P420B was used in immunohistochemistry to investigate the level of interleukin-1??, interleukin-1??, and interleukin-1 receptor antagonist in human testicular normal and abnormal biopsies
|Abu Elheija M,Dyomin V,Ganaiem M,Lunenfeld E,Vardy NS,Huleihel M||Journal of interferon and cytokine research : the official journal of the International Society for Interferon and Cytokine Research (31:401)||2011|
Perfusion with lipopolysaccharide differently affects the secretion of interleukin-1 beta and interleukin-1 receptor antagonist by term and preterm human placentae.
P420B was used in immunohistochemistry to study the secretion of IL-1beta and IL-1Ra by perfused term and preterm human placental tissue and the effects of LPS
|Holcberg G,Amash A,Sapir O,Sheiner E,Levy S,Myatt L,Huleihel M||Placenta (29:593)||2008|
Tumor necrosis factor-related apoptosis-inducing ligand induces neuronal death in a murine model of HIV central nervous system infection.
P420B was used in immunohistochemistry to investigate the role of TRAIL expressed in HIV-1-infected macrophages on neuronal apoptosis
|Miura Y,Misawa N,Kawano Y,Okada H,Inagaki Y,Yamamoto N,Ito M,Yagita H,Okumura K,Mizusawa H,Koyanagi Y||Proceedings of the National Academy of Sciences of the United States of America (100:2777)||2003|
Intrahepatic expression of interleukin-1beta and tumor necrosis factor-alpha in chronic hepatitis C.
P420B was used in immunohistochemistry to investigate the intrahepatic expression of interleukin 1 beta and tumor necrosis factor alpha in liver specimens with chronic hepatitis C
|Dumoulin FL,Leifeld L,Honecker U,Sauerbruch T,Spengler U||The Journal of infectious diseases (180:1704)||1999|
Adoptive transfer of experimental allergic encephalomyelitis after in vitro treatment with recombinant murine interleukin-12. Preferential expansion of interferon-gamma-producing cells and increased expression of macrophage-associated inducible nitric oxide synthase as immunomodulatory mechanisms.
P420B was used in immunohistochemistry to study the effect of interleukin 12 on experimental allergic encephalomyelitis
|Waldburger KE,Hastings RC,Schaub RG,Goldman SJ,Leonard JP||The American journal of pathology (148:375)||1996|
Monocyte:astrocyte interactions regulate MCP-1 expression in both cell types.
P420B was used in blocking or activating experiment to study the role of monocyte-astrocyte interactions in regulating MCP-1 expression in both cell types
|Andjelkovic AV,Kerkovich D,Pachter JS||Journal of leukocyte biology (68:545)||2000|
Activation of nuclear factor-kappa B and gene expression in human endothelial cells by the common haptens nickel and cobalt.
P420B was used in blocking/activating experiment to investigate the effect of nickel chloride and cobalt chloride on the expression of NF-kappa B in human endothelial cells
|Goebeler M,Roth J,Bröcker EB,Sorg C,Schulze-Osthoff K||Journal of immunology (Baltimore, Md. : 1950) (155:2459)||1995|
Induction of IL-3 and granulocyte-macrophage colony-stimulating factor by substance P in bone marrow cells is partially mediated through the release of IL-1 and IL-6.
P420B was used in blocking/activating experiment to investigate the production of interleukin 3 and GM-CSF mediated by substance P in bone marrow mononuclear cells
|Rameshwar P,Ganea D,Gascón P||Journal of immunology (Baltimore, Md. : 1950) (152:4044)||1994|
1,25-Dihydroxyvitamin D3 and its analogues inhibit acute myelogenous leukemia progenitor proliferation by suppressing interleukin-1beta production.
P420B was used in western blot to study the effect of 1,25-dihydroxyvitamin D3 on acute myelogenous leukemia progenitor proliferation and its mechanism
|Peleg S,Qiu H,Reddy S,Harris D,Van Q,Estey EH,Talpaz M,Estrov Z||The Journal of clinical investigation (100:1716)||1997|
Tenidap-modulated proinflammatory cytokine activation of a monocyte cell line.
P420B was used in ELISA to investigate the effect of tenidap on the regulation of inflammation response in monocytes
|Golding S,Emery P,Young SP||Journal of immunology (Baltimore, Md. : 1950) (154:5384)||1995|
Synergistic increases in IL-1 synthesis by the human monocytic cell line THP-1 treated with PAF and endotoxin.
P420B was used in immunocytochemistry to investigate the effect of PAF and endotoxin treatment on interleukin 1 expression
|Barthelson RA,Potter T,Valone FH||Cellular immunology (125:142)||1990|