Invitrogen

IL-1 beta Polyclonal Antibody

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IL-1 beta Antibody in Neutralization (Neu) — Neutralization assay of recombinant Canine IL-1ß/IL-1F2 stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by recombinant Canine IL-1ß/IL-1F2 (20 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine IL-1ß/IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47533). The ND50 is typically 2-10 µg/mL in the presence of concanavalin A (1.25 µg/mL).

Product Details

PA5-47533

Applications	Tested Dilution	Publications
Western Blot (WB)	0.1 µg/mL	-
Flow Cytometry (Flow)	2.5 µg/1x10^6 cells	-
Neutralization (Neu)	2-10 µg/mL	-

Product Specifications
Species Reactivity	Dog
Host/Isotype	Goat / IgG
Class	Polyclonal
Type	Antibody
Immunogen	E. coli-derived recombinant canine IL-1 beta/IL-1F2 Ala114-Ser265 View antigen
Conjugate	Unconjugated
Form	Lyophilized
Concentration	0.2 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 5% trehalose
Contains	No Preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2607649

Product Specific Information

In direct ELISAs, approximately 40% cross-reactivity with recombinant feline IL-1 beta is observed, less than 10% cross-reactivity with recombinant human IL-1 beta, recombinant rhesus macaque IL-1 beta, recombinant equine IL-1 beta, and recombinant porcine IL-1 beta is observed, less than 5% cross-reactivity with recombinant mouse (rm) IL-1 beta, recombinant rat IL-1 beta, and recombinant cotton rat (rcr) IL-1 beta is observed, and less than 1% cross-reactivity with rcrIL-1 alpha, rmIL-1ra, and recombinant canine IL-18 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cytokine; Hematopoietin 1 (H1); IFN beta inducing factor; il 1b; IL 1β; IL-1 beta; IL1 B; IL1β; ILN; Interleukin; Interleukin 1 beta precursor; interleukin-1 beta proprotein; Interleukin-1b; Interleukin1 beta; LAF; Osteoclast activating factor (OAF); Pro interleukin 1 beta

Gene Aliases: IL-1

Entrez Gene ID: (Dog) 403974

Molecular Function: interleukin superfamily