|Tested species reactivity||Pig|
|Published species reactivity||Pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant porcine IL-1 beta|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 1 publications below|
PP425 targets IL-1 beta in WB applications and shows reactivity with Porcine samples.
The PP425 immunogen is recombinant porcine IL-1 beta.
PP425 detects IL-1 beta which has a predicted molecular weight of approximately 18 kDa.
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Development of a cytometric bead array screening tool for the simultaneous detection of pro-inflammatory cytokines in porcine plasma.
PP425 was used in ELISA to develop a cytometric bead array to detect porcine cytokines and acute phase proteins
|Wyns H,Croubels S,Demeyere K,Watteyn A,De Backer P,Meyer E||Veterinary immunology and immunopathology (151:28)||2013|