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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant human IL-2|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 2 publications below|
P600 targets IL-2 in ELISA applications and shows reactivity with Human samples.
The P600 immunogen is recombinant human IL-2.
P600 detects IL-2 which has a predicted molecular weight of approximately 15 kDa.
The P600 IL2 polyclonal antibody has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M600B (biotinylated conjugate of clone BG5). Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
Antibody P600 and biotinylated antibody M600B (clone BG5) have successfully been used in combination with recombinant IL2 protein SIL2 in ELISA applications.
The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A human dual-color enzyme-linked immunospot assay for simultaneous detection of interleukin 2- and interleukin 4-secreting cells.
P600 was used in ELISA to study a human dual-color enzyme-linked immunospot assay for simultaneous detection of interleukin 2- and interleukin 4-secreting cells
|Okamoto Y,Gotoh Y,Shiraishi H,Nishida M||International immunopharmacology (4:149)||2004|
A sensitive ELISPOT assay to detect low-frequency human T lymphocytes.
P600 was used in ELISA to develop a new assay to detect human T lymphocyte subsets
|McCutcheon M,Wehner N,Wensky A,Kushner M,Doan S,Hsiao L,Calabresi P,Ha T,Tran TV,Tate KM,Winkelhake J,Spack EG||Journal of immunological methods (210:149)||1997|
aldesleukin; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor
IL-2; IL2; lymphokine; TCGF