Invitrogen

IL-2 Monoclonal Antibody (S4B6)

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IL-2 Antibody in ELISA (ELISA) — Sandwich ELISA analysis of Mouse IL-2 MAb (Product # MM100) was performed using the Thermo Fisher Scientific Mouse IL-2 Colorimetric ELISA Kit (Product # EMIL2) by loading 50 µl per well of Mouse IL-2 MAb (MM100) in quadruplicate at 0, 50% (2.5 µg/mL), and 100% (5 µg/mL) displacement in a plate reagent and then loading 50 µL per well of Recombinant Mouse IL-2 (Product # SMIL2) in dodecuplicate at 850, 170, 34, and 0 pg/mL across a 5 µg/mL, Mouse IL-2 MAb (Product # MM101) pre-coated plate and incubated for 2 hours at 37°C. The plate was washed then incubated with 100 µL per well of Rat anti-Mouse IL-2 HRP labeled stock at 1:25,000 for 1 hour at 37°C. The plate was washed and incubated with 100 µL per well of TMB substrate solution for 30 minutes at room temperature in the dark. The plate was then stopped with 0.16M sulfuric acid and absorbance was read on a spectrophotometer at 450-550 nm.

Product Details

MM100

Applications	Tested Dilution	Publications
Western Blot (WB)	Assay-dependent	View 1 publication 1 publication
ELISA (ELISA)	Assay-dependent	View 7 publications 7 publications
Functional Assay (FN)	-	View 1 publication 1 publication
Inhibition Assays (IA)	-	View 1 publication 1 publication
Flow Cytometry (Flow)	-	View 1 publication 1 publication
T-Cell Activation (TCA)	-	View 1 publication 1 publication
Immunoprecipitation (IP)	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
Neutralization (Neu)	-	View 4 publications 4 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse
Published species	Fungi, Human, Mouse, Rat
Host/Isotype	Rat / IgG2a
Class	Monoclonal
Type	Antibody
Clone	S4B6
Immunogen	Recombinant mouse IL-2
Conjugate	Unconjugated
Form	Liquid
Concentration	1.0 mg/mL
Purification	Protein G
Storage buffer	PBS
Contains	no preservative
Storage conditions	-20°C
RRID	AB_223523

Product Specific Information

MM100 targets IL-2 in Western blot and ELISA applications and shows reactivity with mouse samples.

The MM100 immunogen is recombinant mouse IL-2.

MM100 detects IL-2 which has a predicted molecular weight of approximately 17 kDa.

This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

mTORC1 Signaling Controls TLR2-Mediated T-Cell Activation by Inducing TIRAP Expression.

Cell reports

Imanishi T,Unno M,Kobayashi W,Yoneda N,Akira S,Saito T

MM100 was used in Functional assay to demonstrate that the mTORC1 signal confers the response to TLR2 signaling by inducing TIRAP expression and that the TIRAP-mTORC1 axis is critical for TLR2-mediated IFN-γ production by effector T cells.

Tue Jul 21 00:00:00 EDT 2020

Regulatory T Cells Suppress Effector T Cell Proliferation by Limiting Division Destiny.

Frontiers in immunology

Dowling MR,Kan A,Heinzel S,Marchingo JM,Hodgkin PD,Hawkins ED

MM100 was used in Inhibition Assays to illustrate the primary effect of Tregs on the magnitude of effector T cell responses is mediated by modifying division destiny of responding cell populations.

Mon Sep 30 00:00:00 EDT 2019

The epigenetic regulator ATF7ip inhibits Il2 expression, regulating Th17 responses.

The Journal of experimental medicine

Sin JH,Zuckerman C,Cortez JT,Eckalbar WL,Erle DJ,Anderson MS,Waterfield MR

MM100 was used in Flow Cytometry to demonstrate a new epigenetic pathway by which IL-2 production is constrained, and this may open up new avenues for modulating its production.

Mon Sep 02 00:00:00 EDT 2019

Discovery of stimulation-responsive immune enhancers with CRISPR activation.

Nature

Simeonov DR,Gowen BG,Boontanrart M,Roth TL,Gagnon JD,Mumbach MR,Satpathy AT,Lee Y,Bray NL,Chan AY,Lituiev DS,Nguyen ML,Gate RE,Subramaniam M,Li Z,Woo JM,Mitros T,Ray GJ,Curie GL,Naddaf N,Chu JS,Ma H,Boyer E,Van Gool F,Huang H,Liu R,Tobin VR,Schumann K,Daly MJ,Farh KK,Ansel KM,Ye CJ,Greenleaf WJ,Anderson MS,Bluestone JA,Chang HY,Corn JE,Marson A

MM100 was used in T-Cell Activation to study the in vivo response of enhancer mutant cells to acute stimulation.

Thu Sep 07 00:00:00 EDT 2017

Phototrophy and starvation-based induction of autophagy upon removal of Gcn5-catalyzed acetylation of Atg7 in Magnaporthe oryzae.

Autophagy

Zhang S,Liang M,Naqvi NI,Lin C,Qian W,Zhang LH,Deng YZ

MM100 was used in Immunoprecipitation to depict a signaling network and regulatory mechanism underlying the autophagy induction by important environmental clues such as light and nutrients.

Thu Aug 03 00:00:00 EDT 2017

Autophagy negatively regulates cancer cell proliferation via selectively targeting VPRBP.

Clinical science (London, England : 1979)

Wang BS,Liu YZ,Yang Y,Zhang Y,Hao JJ,Yang H,Wang XM,Zhang ZQ,Zhan QM,Wang MR

MM100 was used in Immunofluorescence to show that VPRBP was positively correlated with p62 and negatively correlated with LC3B.

Fri Feb 01 00:00:00 EST 2013

Smad4 deficiency in T cells leads to the Th17-associated development of premalignant gastroduodenal lesions in mice.

The Journal of clinical investigation

Hahn JN,Falck VG,Jirik FR

MM100 was used in Western Blotting to evaluate the connection between a chronic increase in gut Th17 cell activity and the development of premalignant lesions of the gastroduodenal region.

Sat Oct 01 00:00:00 EDT 2011

Target cell induced activation of NK cells in vitro: cytokine production and enhancement of cytotoxic function.

Cancer immunology, immunotherapy : CII

Das S,Varalakshmi C,Kumari AL,Patel M,Khar A

MM100 was used in ELISA to investigate the changes of cytokine expression in NK cells after interacting with tumor cells

Mon Oct 01 00:00:00 EDT 2001

Oral administration of L-arginine potentiates allergen-induced airway inflammation and expression of interleukin-5 in mice.

The Journal of pharmacology and experimental therapeutics

Takano H,Lim HB,Miyabara Y,Ichinose T,Yoshikawa T,Sagai M

MM100 was used in ELISA to investigate the effect of L-arginine administration on airway inflammation and cytokine expression

Sat Aug 01 00:00:00 EDT 1998

Long-term exposure to diesel exhaust enhances antigen-induced eosinophilic inflammation and epithelial damage in the murine airway.

Toxicological sciences : an official journal of the Society of Toxicology

Ichinose T,Takano H,Miyabara Y,Sagai M

MM100 was used in ELISA to investigate the effect of diesel exhaust on the damage of mouse airway system

Wed Jul 01 00:00:00 EDT 1998

Identification of the immunodominant T cell epitope of p38, a major egg antigen, and characterization of the epitope-specific Th responsiveness during murine schistosomiasis mansoni.

Journal of immunology (Baltimore, Md. : 1950)

Chen Y,Boros DL

MM100 was used in ELISA to investigate the immunodominant epitope of p38 and the production of Th-derived cytokine during murine Schistosomiasis mansoni infection

Mon Jun 01 00:00:00 EDT 1998

Corticosteroids enhance the capacity of macrophages to induce Th2 cytokine synthesis in CD4+ lymphocytes by inhibiting IL-12 production.

Journal of immunology (Baltimore, Md. : 1950)

DeKruyff RH,Fang Y,Umetsu DT

MM100 was used in blocking/activating experiment to investigate the changes of IL-12 expression modulated by corticosteroids in CD4+ T cells

Sun Mar 01 00:00:00 EST 1998

IL-4 enhances long-term survival of CD28-deficient T cells.

Journal of immunology (Baltimore, Md. : 1950)

Stack RM,Thompson CB,Fitch FW

MM100 was used in blocking/activating experiment to investigate the effect of interleukin 4 on T cel survival

Sun Mar 01 00:00:00 EST 1998

Murine strain differences in airway inflammation caused by diesel exhaust particles.

The European respiratory journal

Miyabara Y,Yanagisawa R,Shimojo N,Takano H,Lim HB,Ichinose T,Sagai M

MM100 was used in ELISA to investigate the strain-specific inflammatory responses against air pollutants

Sun Feb 01 00:00:00 EST 1998

Requirement for in vivo production of IL-4, but not IL-10, in the induction of proliferative suppression by filarial parasites.

Journal of immunology (Baltimore, Md. : 1950)

MacDonald AS,Maizels RM,Lawrence RA,Dransfield I,Allen JE

MM100 was used in blocking/activating experiment to investigate the mechanism for the anti-proliferative activity of filarial parasites

Sun Feb 01 00:00:00 EST 1998

Diesel exhaust particles enhance antigen-induced airway inflammation and local cytokine expression in mice.

American journal of respiratory and critical care medicine

Takano H,Yoshikawa T,Ichinose T,Miyabara Y,Imaoka K,Sagai M

MM100 was used in ELISA to investigate the effect of diesel exhaust particles on airway inflammation and cytokine expression

Tue Jul 01 00:00:00 EDT 1997

A role for nonspecific (cyclosporin A) or specific (monoclonal antibodies to ICAM-1, LFA-1, and IL-10) immunomodulation in the prolongation of skin allografts after antigen-specific pretransplant immunization or transfusion.

Journal of immunology (Baltimore, Md. : 1950)

Gorczynski RM,Wojcik D

MM100 was used in blocking/activating experiment to study the approaches to achieve long-term graft survival in mice

Tue Feb 15 00:00:00 EST 1994

Functionally anergic lpr and gld B220+ T cell receptor (TCR)-alpha/beta+ double-negative T cells express CD28 and respond to costimulation with phorbol myristate acetate and antibodies to CD28 and the TCR.

Journal of immunology (Baltimore, Md. : 1950)

Giese T,Allison JP,Davidson WF

MM100 was used in ELISA to study the mechanism for the immunosuppression of specific T cell subpopulation

Thu Jul 15 00:00:00 EDT 1993

Analysis of IL-2, IL-4, and IFN-gamma-producing cells in situ during immune responses to protein antigens.

Journal of immunology (Baltimore, Md. : 1950)

Bogen SA,Fogelman I,Abbas AK

MM100 was used in immunohistochemistry to investigate the production of interleukin 2 and 4 and interferon gamma in mice immunized with protein antigens

Sat May 15 00:00:00 EDT 1993

Bioinformatics

Protein Aliases: H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; M-IL-2; T-cell growth factor; T-cell growth factor (TCGF); TCGF

Gene Aliases: Il-2; Il2

UniProt ID: (Mouse) P04351

Entrez Gene ID: (Mouse) 16183

Molecular Function: actin or actin-binding cytoskeletal protein non-receptor serine/threonine protein kinase

Function(s)

Process(es)

cytokine activity interleukin-2 receptor binding growth factor activity carbohydrate binding kappa-type opioid receptor binding glycosphingolipid binding

IL-2 Monoclonal Antibody (S4B6)

Product Details

MM100

Western Blot (WB)

ELISA (ELISA)

Functional Assay (FN)

Inhibition Assays (IA)

Flow Cytometry (Flow)

T-Cell Activation (TCA)

Immunoprecipitation (IP)

Immunocytochemistry (ICC/IF)

Neutralization (Neu)

Immunohistochemistry (IHC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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