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Proteintech
IL-27 Polyclonal Antibody
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Product Details
24165-1-AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
IL-27 Fusion Protein Ag21436 (19-243 aa encoded by BC062422)
Conjugate
Form
Liquid
Concentration
0.49 mg/mL
Amount
73.5 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
Immunogen sequence: LLLVQAGVW GFPRPPGRPQ LSLQELRREF TVSLHLARKL LAEVRGQAHR FAESHLPGVN LYLLPLGEQL PDVSLTFQAW RRLSDPERLC FISTTLQPFH ALLGGLGTQG RWTNMERMQL WAMRLDLRDL QRHLRFQVLA AGFNLPEEEE EEEEEEEEER KGLLPGALGS ALQGPAQVSW PQLLSTYRLL HSLELVLSRA VRELLLLSKA GHSVWPLGFP TLSPQP (19-243 aa encoded by BC062422)
Target Information
IL-27, a member of the IL-12 family, is a heterodimeric protein consisting of the p40-related protein Epstein-Barr virus-induced gene 3 (EBI3) non-covalently linked to an IL-12p35-related protein, p28 (also known as IL-30). IL-27 is produced by activated APCs and mature dendritic cells. IL-27 exerts its activities on NK cells and naive CD4+ T cells; mRNA expression analysis of IL-27 receptor components (WSX-1/TCCR and gp130) suggests that IL-27 may also target other cells, including mast cells and monocytes. Binding of IL-27 to WSX-1/gp130 activates JAK1, STAT1, and STAT3 and STAT1/3 phosphorylation. WSX-1/TCCR-deficient mice develop impaired Th1 responses and are more susceptible to infection with L. monocytogenes suggesting that Th1 responses require IL-27. Although activation of WSX-1 is required for the initiation of Th1 responses, it is not necessary for maintaining Th1 responses. IL-27 alone is not able to induce the differentiation of CD4+ T cells into IFN-γ-producing cells, suggesting a role for IL-27 as an initial activator of Th1 responses. An important effect of IL-27 in initiating Th1 responses is the induction of the Th1-specific transcription factor T-bet as well as the suppression of the Th2-specific transcription factor GATA-3. T-bet plays a critical role in Th1 differentiation by its ability to maintain IL-12Rβ2 expression following CD4+ T cell activation. Recent studies indicate that IL-27 has a potent antitumor activity. In vitro, IL-27 has been found to act directly on naive CD8 cells, generating CTL with enhanced granzyme B expression. In vivo, IL-27 has been reported to augment CTL activity, inhibit tumor growth, and induce complete regression of primary and metastatic neuroblastoma tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: IL-27 p28 subunit; IL-27 subunit alpha; IL-27-A; IL-30; IL27-A; ILN; Interleukin; Interleukin-27; Interleukin-27 subunit alpha; Interleukin-30; p28
Gene Aliases: IL-27; IL-27A; IL27; IL27A; IL27p28; IL30; p28
UniProt ID: (Human) Q8NEV9
Entrez Gene ID: (Human) 246778
positive regulation of defense response to virus by host
immune system process
inflammatory response
signal transduction
response to bacterium
positive regulation of interferon-gamma production
regulation of T cell proliferation
innate immune response
regulation of T-helper 1 cell differentiation
regulation of defense response to virus
regulation of T cell activation
response to Gram-positive bacterium
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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