|Tested species reactivity||Mouse|
|Host / Isotype||E.coli|
|Storage buffer||10mM sodium phosphate, pH 7.5|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Control (Ctrl)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Recombinant mouse p28 is a non-glycosylated protein, containing 207 amino acids, with a molecular weight of 23.7 kDa. Purity is typically greater than 97% and endotoxin level less than 1 EU/ug.
The expected biological activity (ED50) is determined by the dose-dependent inhibition of IL-17A expression from mouse CD4+ splenocytes stimulated with TGF-beta and IL-6. 50ng/mL typically inhibits greater than 25% of IL-17A expression.
Reconstitute using sterile water at 0.1 mg/ml and centrifuge prior to opening vial. Gently pipet solution down the sides of the vial. DO NOT VORTEX sample. Store reconstituted material at -20°C and add 0.1% BSA for additional stability.
Amino acid sequence: MFPTDPLSLQ ELRREFTVSL YLARKLLSEV QGYVHSFAES RLPGVNLDLL PLGYHLPNVS LTFQAWHHLS DSERLCFLAT TLRPFPAMLG GLGTQGTWTS SEREQLWAMR LDLRDLHRHL RFQVLAAGFK CSKEEEDKEE EEEEEEEEKK LPLGALGGPN QVSSQVSWPQ LLYTYQLLHS LELVLSRAVR DLLLLSLPRR PGSAWDS.
The p28 subunit of IL-27 (IL-27/p28), also known as Interleukin-30 (IL-30), is a member of the IL-12 family of cytokines. When combined with EBI3 (Epstein-Barr virus induced gene 3), p28 forms a heterodimer known as IL-27. p28 is thought to be a pro-inflammatory cytokine inducing immuno-modulatory effects, but the details of these effects are currently unclear and research is focused on delineating its specific biological functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.