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Invitrogen
IL-29 Polyclonal Antibody
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FIGURE: 1 / 2
IL-29 Antibody (PA5-47165) in Neu
Neutralization of IL-29 in HepG2 human hepatocellular carcinoma cell line. Samples were incubated in IL-29 polyclonal antibody (Product # PA5-47165). Recombinant Human IL‚29/IFN‚λ1 reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IL‚29/IFN‚λ1 (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‚29/... View More
Product Details
PA5-47165
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-29/IFN-lambda 1 Gly20-Thr200
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant human (rh) IL-28A and rhIL-28B is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
IFN-lambdas constitute a novel family of interferons that mediate the induction of anti-viral protection in a wide variety of cells. There are three members in this family, namely lambda1, lambda2, and lambda3, which are also known as IL-29, IL-28A, and IL-28B, respectively. Similar to type I IFNs, IFN-lambdas activate an intracellular signaling to promote gene expression. IFN-lambdas mediate their anti-viral protection through a class II cytokine receptor complex composed of two essential receptor proteins, CRF2-12/IFN-lambdaR1, which is unique to IFN-lambdas, and CFR2-4/IL-10R2, which is shared with IL-10, IL-22, and IL-26 receptors. The IFN-lambdas, IL-28 and IL-29, have recently been reported to prime dendritic cells to induce proliferation of Foxp3-bearing regulatory T cells. DCs that differentiate in response to IFN-lambdas express high levels of class I and II MHC gene products, but low levels of costimulatory molecules. Moreover, these cells can specifically induce IL-2-dependent proliferation of CD4+CD25+FOXP3+ T cells with contact-dependent suppressive activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytokine; Cytokine Zcyto21; FNL1; IFN lambda1; IFN-lambda-1; IFN-lambda1; cytokine; IL-29; ILN; Interferon lambda-1; Interferon lambda1; Interleukin; interleukin 29 (interferon, lambda 1); Interleukin-29; produced during viral infection; involved in Jak/STAT pathway
Gene Aliases: IFNL1; IL-29; IL29; ZCYTO21
UniProt ID: (Human) Q8IU54
Entrez Gene ID: (Human) 282618
negative regulation of type 2 immune response
JAK-STAT cascade
negative regulation of cell proliferation
negative regulation of interleukin-13 production
negative regulation of interleukin-5 production
positive regulation of interferon-gamma production
type III interferon-mediated signaling pathway
positive regulation of tyrosine phosphorylation of STAT protein
negative regulation of memory T cell differentiation
innate immune response
positive regulation of MHC class I biosynthetic process
negative regulation of T cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of JAK-STAT cascade
positive regulation of immune response
defense response to virus
cellular response to virus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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