|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 19 amino acid peptide from near the amino terminus of human IL-33.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||20 ug/ml|
|Immunofluorescence (IF)||20 ug/ml|
|Immunohistochemistry (IHC)||20 ug/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
Despite its predicted molecular weight, IL-33 often migrates at a higher molecular weight in SDS-PAGE. A suggested positive control is human lymph node tissue lysate.
PA5-20397 can be used with blocking peptide PEP-0514.
Interleukin-33 (IL-33) is a recently identified member of the IL-1 family of cytokines whose other members include IL-1-a/beta, IL-1Ra and IL-18. Its receptor has been shown to be ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling and IL-1R accessory protein (IL-1RAcP). Receptor binding of IL-33 activates NF-kappa-B and MAP kinases and induces the expression of TH2-associated cytokines such as IL-4, IL-5 and IL-6. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Human 'brite/beige' adipocytes develop from capillary networks, and their implantation improves metabolic homeostasis in mice.
PA5-20397 was used in immunocytochemistry to study human 'brite/beige' adipocytes and how they develop from capillary networks and how their implantation improves metabolic homeostasis in mice
|Min SY,Kady J,Nam M,Rojas-Rodriguez R,Berkenwald A,Kim JH,Noh HL,Kim JK,Cooper MP,Fitzgibbons T,Brehm MA,Corvera S||Nature medicine (22:312)||2016|