Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
0.1-0.2 µg/mL | - |
Immunohistochemistry (IHC) |
- | View 1 publication 1 publication |
Flow Cytometry (Flow) |
- | View 3 publications 3 publications |
ELISA (ELISA) |
0.5-2.0 µg/mL | View 1 publication 1 publication |
Neutralization (Neu) |
- | View 1 publication 1 publication |
In vitro Assay (IV) |
- | View 14 publications 14 publications |
Immunostaining (IS) |
- | View 2 publications 2 publications |
Product Specifications | |
---|---|
Species Reactivity |
Mouse |
Published species |
Human, Mouse |
Host/Isotype |
Rabbit |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
E.coli-derived Recombinant Murine IL-4 |
Conjugate |
Unconjugated |
Form |
Lyophilized |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS |
Contains |
no preservative |
Storage conditions |
-20°C |
AA Sequence of recombinant protein: MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-4. Anti-Murine IL-4-specific antibody was purified by affinity chromatography employing an immobilized Murine IL-4 matrix.
Sandwich ELISA: To detect Murine IL-4 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine IL-4 (500-P54Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IL-4.
Western Blot: To detect Murine IL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine IL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; BSF-1; H-IL-4; IGG1 induction factor; IL-4; ILN; Interleukin; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402
Gene Aliases: BSF-1; Il-4; Il4
UniProt ID: (Mouse) P07750
Entrez Gene ID: (Mouse) 16189
Molecular Function:
interleukin superfamily
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