Western blot analysis of IL-6 was performed by loading 20µg of LPS-treated THP-1 whole cell lysates in non-reducing sample buffer and 8ul PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel (Product #WT4202BOX). Proteins were transferred to a nitrocellulose membrane using the G2 Fast Blotter (Product # 62288) and blocked with 5% Milk/TBST for at least 1 hour at room temperature. IL-6 was detected using a IL-6 rabbit polyclonal antibody (Product # P620) at a dilution of 1:1000 in blocking buffer overnight at 4°C on a rocking platform, followed by a HRP conjugated secondary antibody (Product # A16104) at a dilution of 1:5000 for at least 1 hour at room temperature. Chemiluminescent detection was performed using SuperSignal West Dura Extended Duration Substrate (Product # 34076) and the myECL Imager (Product # 62236).
|Tested species reactivity||Human|
|Published species reactivity||Pig, Rat, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant human IL-6.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50-1:200|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
P620 detects IL-6 in WB, Flow and ELISA applications with human samples.
The P620 immunogen is recombinant human IL-6.
P620 detects IL-6 which has a predicted molecular weight of approximately 22 kDa.
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Combined immunogene therapy of IL-6 and IL-15 enhances anti-tumor activity through augmented NK cytotoxicity.
P620 was used in immunocytochemistry to study the ability of combined IL-6 and IL-15 immunogene therapy to enhance natural killer cell cytotoxicity and anti-tumor activity
|Lin CY,Chuang TF,Liao KW,Huang YJ,Pai CC,Chu RM||Cancer letters (272:285)||2008|
Human mast cell activation with virus-associated stimuli leads to the selective chemotaxis of natural killer cells by a CXCL8-dependent mechanism.
P620 was used in ELISA to study the role of human mast cells in the recruitment of natural killer cells
|Burke SM,Issekutz TB,Mohan K,Lee PW,Shmulevitz M,Marshall JS||Blood (111:5467)||2008|
Secretion of proinflammatory cytokines by epithelial cells in response to Chlamydia infection suggests a central role for epithelial cells in chlamydial pathogenesis.
P620 was used in ELISA to investigate the important roles of epithelial cells in inflammatory response to chlamydia
|Rasmussen SJ,Eckmann L,Quayle AJ,Shen L,Zhang YX,Anderson DJ,Fierer J,Stephens RS,Kagnoff MF||The Journal of clinical investigation (99:77)||1997|
Entamoeba histolytica trophozoites induce an inflammatory cytokine response by cultured human cells through the paracrine action of cytolytically released interleukin-1 alpha.
P620 was used in ELISA to study the effect of Entamoeba histolytica trophozoites on cytokine expression
|Eckmann L,Reed SL,Smith JR,Kagnoff MF||The Journal of clinical investigation (96:1269)||1995|
Serum IL-4, IL-10 and IL-6 levels in inflammatory arthritis.
P620 was used in ELISA to investigate the interleukin expression and secretion in patients with inflalmnatory arthritis
|Cicuttini FM,Byron KA,Maher D,Wootton AM,Muirden KD,Hamilton JA||Rheumatology international (14:201)||1995|
A distinct array of proinflammatory cytokines is expressed in human colon epithelial cells in response to bacterial invasion.
P620 was used in ELISA to investigate the expression of proinflammatory cytokines in human colon epithelial cells
|Jung HC,Eckmann L,Yang SK,Panja A,Fierer J,Morzycka-Wroblewska E,Kagnoff MF||The Journal of clinical investigation (95:55)||1995|
Interleukin-6 fused to an anti-idiotype antibody in a vaccine increases the specific humoral immune response against CA125+ (MUC-16) ovarian cancer.
P620 was used in blocking or activating experiment to study the capacity of IL-6 fused to an anti-idiotype antibody in a vaccine to increase the specific humoral immune response against CA125+ ovarian cancer
|Reinartz S,Hombach A,Köhler S,Schlebusch H,Wallwiener D,Abken H,Wagner U||Cancer research (63:3234)||2003|
Interleukin-6 stimulates neutrophil production of platelet-activating factor.
P620 was used in blocking/activating experiment to study the effect of interleukin 6 on the platelet-activating factor production by neutrophils
|Biffl WL,Moore EE,Moore FA,Barnett CC,Silliman CC,Peterson VM||Journal of leukocyte biology (59:569)||1996|
Soluble IgA and IgG aggregates are catabolized by cultured rat mesangial cells and induce production of TNF-alpha and IL-6, and proliferation.
P620 was used in blocking/activating experiment to study the mechanism for the mesangial deposits of IgA immune complexes in IgA nephropathy
|Gómez-Guerrero C,López-Armada MJ,González E,Egido J||Journal of immunology (Baltimore, Md. : 1950) (153:5247)||1994|
Changes in macrophages in spleen and lymph nodes during acute African swine fever: expression of cytokines.
P620 was used in immunohistochemistry to study the role of cytokine expression by macrophages in spleen and lymph nodes in the pathogenesis of African swine fever
|Salguero FJ,Ruiz-Villamor E,Bautista MJ,Sánchez-Cordón PJ,Carrasco L,Gómez-Villamandos JC||Veterinary immunology and immunopathology (90:11)||2002|
Localization of cytokines in cholesteatoma tissue.
P620 was used in immunohistochemistry to investigate the distribution of various cytokines in cholesteatoma tissue
|Marenda SA,Aufdemorte TB||Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery (112:359)||1995|
Differential expression and ligand-induced modulation of the human interleukin-6 receptor on interleukin-6-responsive cells.
P620 was used in immunoprecipitation to investigate the differential expression and regulation of interleukin 6 receptor
|Schwabe M,Zhao J,Kung HF||The Journal of biological chemistry (269:7201)||1994|