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          • Primary Antibodies ›
          • IL-6 Antibodies

          Invitrogen

          IL-6 Polyclonal Antibody

          Advanced Verification
          11 Published Figures
          26 References
          View all (207) IL-6 antibodies

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          Cite IL-6 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (6)
          • Published Figures (11)
          • Advanced Verification (2)
          IL-6 Antibody in Immunocytochemistry (ICC/IF)
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          IL-6 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 19

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          IL-6 Antibody (P620) in ICC/IF

          Immunofluorescence analysis of IL-6 was performed using 70% confluent log phase HUVEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with IL-6 Polyclonal Antibody (Product # P620) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          IL-6 Antibody in Immunocytochemistry (ICC/IF)
          IL-6 Antibody in Western Blot (WB)
          IL-6 Antibody in Western Blot (WB)
          IL-6 Antibody in Flow Cytometry (Flow)
          IL-6 Antibody in Flow Cytometry (Flow)
          IL-6 Antibody in ELISA (ELISA)
          IL-6 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          IL-6 Antibody in Western Blot (WB)
          IL-6 Antibody in Western Blot (WB)
          IL-6 Antibody in Western Blot (WB)
          IL-6 Antibody in Western Blot (WB)
          IL-6 Antibody in ELISA (ELISA)
          IL-6 Antibody in ELISA (ELISA)
          IL-6 Antibody in Neutralization (Neu)
          IL-6 Antibody in Neutralization (Neu)
          IL-6 Antibody in Neutralization (Neu)
          IL-6 Antibody in Neutralization (Neu)
          IL-6 Antibody
          IL-6 Antibody

          Product Details

          P620

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          View 8 publications 8 publications

          Immunohistochemistry (IHC)

          -
          View 2 publications 2 publications

          Immunohistochemistry (PFA fixed) (IHC (PFA))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          1:100
          View 2 publications 2 publications

          Flow Cytometry (Flow)

          1:50-1:200
          -

          ELISA (ELISA)

          1:50-1:5,000
          View 6 publications 6 publications

          Immunoprecipitation (IP)

          -
          View 1 publication 1 publication

          Neutralization (Neu)

          -
          View 6 publications 6 publications
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Human, Mouse, Pig, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant human IL-6.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1.0 mg/mL

          Purification

          Protein G

          Storage buffer

          PBS

          Contains

          no preservative

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_223481

          Product Specific Information

          P620 detects IL-6 in WB, Flow and ELISA applications with human samples.



          The P620 immunogen is recombinant human IL-6.



          P620 detects IL-6 which has a predicted molecular weight of approximately 22 kDa.



          This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

          Target Information

          IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF-2; CDF; CTL differentiation factor; H-IL-6; Hybridoma growth factor; hybridoma growth factor peptide; IFN-beta-2; IL-6; IL6; ILN; interferon 6 precursor; Interferon beta-2; Interleukin; interleukin 6; interleukin BSF-2; Interleukin HP-1; Interleukin-6; Interleukin6; M-IL-6; R-IL-6; unnamed protein product

          View more View less

          Gene Aliases: BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6; IL6

          View more View less

          UniProt ID: (Human) P05231, (Mouse) P08505

          View more View less

          Entrez Gene ID: (Human) 3569, (Mouse) 16193

          View more View less

          Function(s)
          cytokine activity interleukin-6 receptor binding protein binding growth factor activity identical protein binding receptor binding
          Process(es)
          neutrophil apoptotic process germinal center B cell differentiation hepatic immune response neutrophil mediated immunity monocyte chemotaxis positive regulation of immunoglobulin production positive regulation of acute inflammatory response positive regulation of leukocyte chemotaxis acute-phase response inflammatory response immune response humoral immune response JAK-STAT cascade positive regulation of cell proliferation negative regulation of cell proliferation vascular endothelial growth factor production regulation of vascular endothelial growth factor production positive regulation of vascular endothelial growth factor production positive regulation of gene expression positive regulation of epithelial to mesenchymal transition negative regulation of lipid storage response to activity cytokine-mediated signaling pathway cell differentiation platelet activation endocrine pancreas development neuron projection development response to peptidoglycan negative regulation of chemokine production positive regulation of chemokine production positive regulation of interleukin-1 beta production positive regulation of interleukin-10 production positive regulation of interleukin-17 production positive regulation of interleukin-21 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production negative regulation of collagen biosynthetic process positive regulation of peptidyl-serine phosphorylation maintenance of blood-brain barrier positive regulation of T cell proliferation glucose homeostasis positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of MAPK cascade negative regulation of fat cell differentiation positive regulation of osteoblast differentiation positive regulation of translation regulation of angiogenesis negative regulation of bone resorption positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JAK-STAT cascade positive regulation of smooth muscle cell proliferation positive regulation of peptidyl-tyrosine phosphorylation negative regulation of neurogenesis regulation of insulin secretion defense response to Gram-negative bacterium defense response to Gram-positive bacterium positive regulation of B cell activation regulation of protein transport positive regulation of lymphocyte activation response to glucocorticoid defense response to virus positive regulation of glial cell proliferation T follicular helper cell differentiation regulation of astrocyte activation neuron cellular homeostasis glucagon secretion regulation of glucagon secretion interleukin-6-mediated signaling pathway cellular response to hydrogen peroxide cellular response to lipopolysaccharide T-helper 17 cell lineage commitment hepatocyte proliferation positive regulation of extracellular matrix disassembly inflammatory response to wounding liver regeneration receptor signaling pathway via STAT cellular response to virus regulation of neuroinflammatory response positive regulation of neuroinflammatory response positive regulation of cytokine production involved in inflammatory response positive regulation of platelet aggregation positive regulation of apoptotic DNA fragmentation positive regulation of pri-miRNA transcription from RNA polymerase II promoter regulation of microglial cell activation positive regulation of STAT cascade positive regulation of leukocyte adhesion to vascular endothelial cell positive regulation of T-helper 2 cell cytokine production negative regulation of primary miRNA processing negative regulation of interleukin-1-mediated signaling pathway positive regulation of type B pancreatic cell apoptotic process myeloid cell homeostasis negative regulation of protein kinase activity regulation of cell shape response to wounding cell growth positive regulation of protein import into nucleus, translocation T cell activation regulation of cell proliferation positive regulation of tyrosine phosphorylation of Stat3 protein defense response to protozoan regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of chemokine biosynthetic process regulation of circadian sleep/wake cycle, non-REM sleep positive regulation of nitric oxide biosynthetic process cell redox homeostasis positive regulation of T-helper 2 cell differentiation positive regulation of neuron differentiation negative regulation of gluconeogenesis positive regulation of DNA replication muscle cell cellular homeostasis negative regulation of hormone secretion negative regulation of muscle organ development positive regulation of epithelial cell proliferation negative regulation of cytokine secretion positive regulation of immunoglobulin secretion positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of protein kinase B signaling positive regulation of transmission of nerve impulse branching involved in salivary gland morphogenesis epithelial cell proliferation involved in salivary gland morphogenesis positive regulation of ERK1 and ERK2 cascade cellular response to interleukin-1 cellular response to tumor necrosis factor negative regulation of neuron death
          It has to be done as per old AB suggested Products section.

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