|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant human IL6, expressed in E.coli.|
|Storage buffer||whole serum diluted in 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:400-1:800|
|Immunohistochemistry (Paraffin) (IHC (P))||1:400-1:800|
|Radioimmune Assays (RIA)||1:4000-1:8000|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-26811 detects IL6 from human samples.
PA1-26811 has been successfully used in ELISA, immunohistochemistry (frozen, paraffin), immunoprecipitation, radioimmune assay, and Western blot procedures.
The PA1-26811 immunogen is recombinant human IL6, expressed in E.coli.
Store at 4°C for short term. For extended storage store at -20°C or below; avoid freeze/thaw cycles.
Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infaction and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. It acts upon a variety of cells including fibroblasts, myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.