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Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
Assay-dependent | View 2 publications 2 publications |
Immunohistochemistry (IHC) |
- | View 1 publication 1 publication |
ELISA (ELISA) |
Assay-dependent | - |
Neutralization (Neu) |
- | View 2 publications 2 publications |
Product Specifications | |
---|---|
Species Reactivity |
Mouse |
Published species |
Mouse |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Recombinant mouse IL-6 |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1.0 mg/mL |
Purification |
Protein G |
Storage buffer |
PBS |
Contains |
no preservative |
Storage conditions |
-20°C |
RRID |
AB_223488 |
PM626 targets IL-6 in ELISA, and WB applications and shows reactivity with mouse samples.
The PM626 immunogen is recombinant mouse IL-6.
PM626 detects IL-6 which has a predicted molecular weight of approximately 22 kDa.
This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).
Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: B-cell hybridoma growth factor; H-IL-6; IL-6; ILN; Interleukin; Interleukin HP-1; Interleukin-6; Interleukin6; M-IL-6; R-IL-6
Gene Aliases: Il-6; Il6
UniProt ID: (Mouse) P08505
Entrez Gene ID: (Mouse) 16193
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