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          • Primary Antibodies ›
          • IL-6 Antibodies

          Huabio

          IL-6 Polyclonal Antibody

          View all (230) IL-6 antibodies

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          Cite IL-6 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (4)
          IL-6 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          IL-6 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          IL-6 Antibody (R1412-2) in ICC/IF

          ICC staining of IL-6 in Siha cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1412-2, 1:100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          IL-6 Antibody in Immunocytochemistry (ICC/IF)
          IL-6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          IL-6 Antibody in Western Blot (WB)
          IL-6 Antibody in Flow Cytometry (Flow)
          IL-6 Polyclonal Antibody

          Product Details

          R1412-2

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:200
          -

          Flow Cytometry (Flow)

          1:50
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein within mouse IL6 aa 1-211.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.4, with 0.2% BSA, 40% glycerol

          Contains

          0.05% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Target Information

          IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF-2; CDF; CTL differentiation factor; H-IL-6; Hybridoma growth factor; hybridoma growth factor peptide; IFN-beta-2; IL-6; IL6; ILN; interferon 6 precursor; Interferon beta-2; Interleukin; interleukin 6; Interleukin 6 (interferon, beta 2); interleukin BSF-2; Interleukin HP-1; Interleukin-6; Interleukin6; M-IL-6; R-IL-6; unnamed protein product

          View more View less

          Gene Aliases: BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6; IL6; ILg6

          View more View less

          UniProt ID: (Human) P05231, (Rat) P20607, (Mouse) P08505

          View more View less

          Entrez Gene ID: (Human) 3569, (Rat) 24498, (Mouse) 16193

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          Function(s)
          cytokine activity interleukin-6 receptor binding protein binding growth factor activity identical protein binding receptor binding
          Process(es)
          neutrophil apoptotic process response to yeast myeloid cell homeostasis hepatic immune response positive regulation of acute inflammatory response negative regulation of protein kinase activity acute-phase response inflammatory response aging positive regulation of cell proliferation negative regulation of cell proliferation regulation of cell shape response to heat response to cold response to wounding response to mechanical stimulus response to bacterium regulation of vascular endothelial growth factor production positive regulation of gene expression response to auditory stimulus response to organic cyclic compound cell growth cytokine-mediated signaling pathway response to caffeine endocrine pancreas development neuron projection development response to nutrient levels response to peptidoglycan response to lipopolysaccharide positive regulation of chemokine production positive regulation of interleukin-6 production response to insulin negative regulation of collagen biosynthetic process positive regulation of peptidyl-serine phosphorylation positive regulation of protein import into nucleus, translocation response to cytokine wound healing positive regulation of T cell proliferation T cell activation regulation of cell proliferation response to drug positive regulation of tyrosine phosphorylation of Stat3 protein glucose homeostasis defense response to protozoan regulation of apoptotic process positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process response to amino acid positive regulation of MAPK cascade negative regulation of chemokine biosynthetic process regulation of circadian sleep/wake cycle, non-REM sleep positive regulation of nitric oxide biosynthetic process cell redox homeostasis positive regulation of T-helper 2 cell differentiation positive regulation of neuron differentiation negative regulation of gluconeogenesis positive regulation of translation positive regulation of DNA replication positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JAK-STAT cascade response to antibiotic muscle cell cellular homeostasis bone remodeling negative regulation of hormone secretion negative regulation of muscle organ development positive regulation of smooth muscle cell proliferation positive regulation of epithelial cell proliferation negative regulation of cytokine secretion positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium defense response to Gram-positive bacterium positive regulation of B cell activation positive regulation of immunoglobulin secretion positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of NF-kappaB transcription factor activity response to glucocorticoid response to calcium ion response to electrical stimulus defense response to virus positive regulation of protein kinase B signaling positive regulation of transmission of nerve impulse branching involved in salivary gland morphogenesis epithelial cell proliferation involved in salivary gland morphogenesis glucagon secretion interleukin-6-mediated signaling pathway cellular response to hydrogen peroxide positive regulation of ERK1 and ERK2 cascade cellular response to lipopolysaccharide cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to dexamethasone stimulus T-helper 17 cell lineage commitment negative regulation of neuron death germinal center B cell differentiation neutrophil mediated immunity monocyte chemotaxis positive regulation of immunoglobulin production positive regulation of leukocyte chemotaxis immune response humoral immune response JAK-STAT cascade vascular endothelial growth factor production positive regulation of vascular endothelial growth factor production positive regulation of epithelial to mesenchymal transition negative regulation of lipid storage response to activity cell differentiation platelet activation negative regulation of chemokine production positive regulation of interleukin-1 beta production positive regulation of interleukin-10 production positive regulation of interleukin-17 production positive regulation of interleukin-21 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production maintenance of blood-brain barrier negative regulation of fat cell differentiation positive regulation of osteoblast differentiation regulation of angiogenesis negative regulation of bone resorption negative regulation of neurogenesis regulation of insulin secretion regulation of protein transport positive regulation of lymphocyte activation positive regulation of glial cell proliferation T follicular helper cell differentiation regulation of astrocyte activation neuron cellular homeostasis regulation of glucagon secretion hepatocyte proliferation positive regulation of extracellular matrix disassembly inflammatory response to wounding liver regeneration receptor signaling pathway via STAT cellular response to virus regulation of neuroinflammatory response positive regulation of neuroinflammatory response positive regulation of cytokine production involved in inflammatory response positive regulation of platelet aggregation positive regulation of apoptotic DNA fragmentation positive regulation of pri-miRNA transcription from RNA polymerase II promoter regulation of microglial cell activation positive regulation of STAT cascade positive regulation of leukocyte adhesion to vascular endothelial cell positive regulation of T-helper 2 cell cytokine production negative regulation of primary miRNA processing negative regulation of interleukin-1-mediated signaling pathway positive regulation of type B pancreatic cell apoptotic process
          It has to be done as per old AB suggested Products section.

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