Western blot analysis of ILK using A) 30 µg Neuro2A whole cell extract (B) 30 µg C8D30 whole cell extract (C) 30 µg NIH-3T3 whole cell extract (D) 30 µg Raw 264.7 whole cell extract and E) 30 µg C2Cl2 whole cell extract. Samples were loaded onto a 10% SDS-PAGE gel and probed with an ILK polyclonal antibody (Product # PA5-27484) at a dilution of 1:1000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 257 of Human ILK|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-27484 targets ILK in WB applications and shows reactivity with Human samples.
The PA5-27484 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 257 of Human ILK.
Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
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