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Western blot analysis of ILK using A) 30 µg Neuro2A whole cell extract (B) 30 µg C8D30 whole cell extract (C) 30 µg NIH-3T3 whole cell extract (D) 30 µg Raw 264.7 whole cell extract and E) 30 µg C2Cl2 whole cell extract. Samples were loaded onto a 10% SDS-PAGE gel and probed with an ILK polyclonal antibody (Product # PA5-27484) at a dilution of 1:1000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 257 of Human ILK|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-27484 targets ILK in WB applications and shows reactivity with Human samples.
The PA5-27484 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 257 of Human ILK.
Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
59 kDa serine/threonine-protein kinase; epididymis secretory protein Li 28; integrin-linked kinase; integrin-linked kinase-2; integrin-linked protein kinase; P59
HEL-S-28; ILK; ILK-1; ILK-2; ILK1; ILK2; P59; p59ILK