|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the internal region of the human, mouse and rat ING4 proteins|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The ING4 gene belongs to the ING (Inhibitor of Growth) family of tumor suppressors. All five members of the ING protein family identified to date contain a highly conserved plant homeodomain (PHD) finger motif at the C-terminal end, which is found in transcription factors that modulate chromatin structure. ING proteins have been shown to form transcriptional complexes leading to activation of responsive genes that mediate a wide range of cellular functions, including growth arrest, DNA repair, gene transcription, apoptosis, and senescence. The candidate tumor suppressor ING4 has a role in brain tumor pathogenesis, and is involved in regulating tumor growth and angiogenesis. Expression of ING4 is reduced in gliomas compared to normal brain tissue, and ING4 reduction correlates with progression from lower to higher grades of tumors. ING4 physically interacts with the p65 (RelA) subunit of NF-kB and represses transcription of NF-kB responsive genes. Inactivating mutations in ING4 transcripts have been described in various human cancer cell lines, and deletion of the ING4 locus has been detected in 10-20% of human breast cancer cell lines and tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Reduced ING4 Expression Is Associated with the Malignancy of Human Bladder.
40-7700 was used in immunohistochemistry (paraffin) and western blot to examine the effects of ING4 expression in human bladder cancer.
|Wang Y,Wang T,Han Y,Wu H,Zhao W,Tong D,Wei L,Zhong Z,An R,Wang Y||Urologia internationalis (94:464)||2015|
|Human||Not Cited||Down-regulation of ING4 is associated with initiation and progression of lung cancer.||Wang QS,Li M,Zhang LY,Jin Y,Tong DD,Yu Y,Bai J,Huang Q,Liu FL,Liu A,Lee KY,Fu SB||Histopathology (57:271)||2010|