Invitrogen

INHA Polyclonal Antibody

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INHA Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded rat brain using INHA Polyclonal antibody (Product # PA5-95909) at a dilution of 1:200 (40x lens).

INHA Antibody in Western Blot (WB) — Western blot analysis of extracts of rat testis, using INHA Polyclonal antibody (Product # PA5-95909) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Product Details

Tested Applications

Dilution

Immunohistochemistry (Paraffin) (IHC (P))

1:50 - 1:200

Western Blot (WB)

1:500 - 1:2000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 19-366 of human INHA.

Conjugate

Unconjugated

Form

Liquid

Concentration

2.14 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Immunogen sequence: CQGLELAREL VLAKVRALFL DALGPPAVTR EGGDPGVRRL PRRHALGGFT HRGSEPEEEE DVSQAILFPA TDASCEDKSA ARGLAQEAEE GLFRYMFRPS QHTRSRQVTS AQLWFHTGLD RQGTAASNSS EPLLGLLALS PGGPVAVPMS LGHAPPHWAV LHLATSALSL LTHPVLVLLL RCPLCTCSAR PEATPFLVAH TRTRPPSGGE RARRSTPLMS WPWSPSALRL LQRPPEEPAA HANCHRVALN ISFQELGWER WIVYPPSFIF HYCHGGCGLH IPPNLSLPVP GAPPTPAQPY SLLPGAQPCC AALPGTMRPL HVRTTSDGGY SFKYETVPNL LTQHCACI; Positive Samples: Rat testis

Target Information

The inhibin alpha subunit joins either the beta A or beta B subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumor-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. However, in prostate cancer, expression of the inhibin alpha-subunit gene was suppressed and was not detectable in poorly differentiated tumor cells. Furthermore, because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.