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Invitrogen
INSIG1 Polyclonal Antibody
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FIGURE: 1 / 2
INSIG1 Antibody (PA1-16988) in ICC/IF
Immunocytochemistry analysis of INSIG1 in HepG2 cells. Samples were incubated with INSIG1 polyclonal antibody (Product # PA1-16988) followed by Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Product Details
PA1-16988
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide representing an internal region of the human INSIG-1 protein (between residues 1-100).
Conjugate
Form
Liquid
Concentration
1.02 mg/mL
Amount
102 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 30% glycerol
Contains
0.1% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody does not cross-react with Insig2.
Suggested positive control: HepG2 lysate, antigen standard for INSIG1 (transient overexpression lysate).
Target Information
Oxysterols regulate cholesterol homeostasis through liver X receptor (LXR) and sterol regulatory element-binding protein (SREBP) mediated signaling pathway. This gene is an insulin-induced gene. It encodes an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. This protein binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ER located; growth response protein (CL-6); Immediate-early protein CL-6; INSIG-1; INSIG-1 membrane protein; Insulin-induced gene 1 protein; Insulin-induced growth response protein CL-6; MGC1405; unnamed protein product
Gene Aliases: Cl-6; CL6; INSIG1
UniProt ID: (Human) O15503, (Rat) Q08755
Entrez Gene ID: (Human) 3638, (Rat) 64194
NLS-bearing protein import into nucleus
lipid metabolic process
triglyceride metabolic process
cholesterol biosynthetic process
response to sterol depletion
steroid metabolic process
cholesterol metabolic process
negative regulation of steroid biosynthetic process
sterol biosynthetic process
cellular response to insulin stimulus
SREBP signaling pathway
cellular response to sterol
SREBP-SCAP complex retention in endoplasmic reticulum
inner ear morphogenesis
middle ear morphogenesis
cholesterol homeostasis
negative regulation of cholesterol biosynthetic process
positive regulation of cholesterol biosynthetic process
negative regulation of fat cell differentiation
negative regulation of fatty acid biosynthetic process
protein maturation
palate development
cranial suture morphogenesis
negative regulation of protein exit from endoplasmic reticulum
negative regulation of cargo loading into COPII-coated vesicle
response to estradiol
response to lipid
response to peptide hormone
response to fatty acid
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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