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INSIG1 Antibody (PA5-109862) in WB
Western blot analysis of INSIG1 in extracts of Rat brain. Samples were incubated with INSIG1 Polyclonal antibody (Product # PA5-109862) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 150s.
Product Details
PA5-109862
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human INSIG1 (NP_005533.2).
Conjugate
Form
Liquid
Concentration
4.56 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2855273
Product Specific Information
Immunogen sequence: LWWTFDRSRS GLGLGITIAF LATLITQFLV YNGVYQYTSP DFLYIRSWLP CIFFSGGVTV GNIGRQLAMG VPEKPHSD
Target Information
Oxysterols regulate cholesterol homeostasis through liver X receptor (LXR) and sterol regulatory element-binding protein (SREBP) mediated signaling pathway. This gene is an insulin-induced gene. It encodes an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. This protein binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: growth response protein (CL-6); Immediate-early protein CL-6; INSIG-1; INSIG-1 membrane protein; Insulin-induced gene 1 protein; Insulin-induced growth response protein CL-6; MGC1405
Gene Aliases: CL-6; CL6; INSIG1
UniProt ID: (Human) O15503, (Rat) Q08755
Entrez Gene ID: (Human) 3638, (Rat) 64194
triglyceride metabolic process
cholesterol biosynthetic process
metabolic process
cell proliferation
negative regulation of steroid biosynthetic process
SREBP signaling pathway
inner ear morphogenesis
middle ear morphogenesis
negative regulation of fat cell differentiation
negative regulation of fatty acid biosynthetic process
palate development
cranial suture morphogenesis
negative regulation of cargo loading into COPII-coated vesicle
response to sterol depletion
cholesterol metabolic process
sterol biosynthetic process
response to estradiol
cellular response to insulin stimulus
response to lipid
response to peptide hormone
response to fatty acid
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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