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Invitrogen
IP3 Receptor 2 Polyclonal Antibody
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FIGURE: 1 / 7
IP3 Receptor 2 Antibody (PA1-904) in ICC/IF
Immunofluorescence analysis of IP3 Receptor II was done on 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with IP3 Receptor II Rabbit Polyclonal Antibody (Product # PA1-904) at 2 µg/mL in 0.1% BSA and incubated for 3 hours ... View More
Product Details
PA1-904
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Amphibian,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: E(317) L N P D Y R D A Q N E G K T V R D(334).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-904 detects inositol 1,4,5-trisphosphate receptor type-II (IP3R-II) from rat and mouse tissues.
PA1-904 has been successfully used in immunohistochemistry, immunofluorescence and immunocytochemistry procedures. Immunohistochemical staining of IP3R-II in rat brain with PA1-904 results in staining of the hippocampus, corpus collosum, and cerebellum. This product fails to detect IP3R-II in Western blot procedures.
PA1-904 immunizing peptide corresponds to amino acid residues 317-334 from rat IP3R-II. PA1-904 immunizing peptide (Cat. # PEP-024) is available for use in neutralization and control experiments.
Target Information
Inositol 1,4,5-trisphosphate (IP3) is a second messenger for many growth factors, hormones, and neuro-transmitters. Upon binding to IP3R, IP3 triggers the release of intracellular, luminal calcium to the cytosol. Functional IP3R is a homo- or heterotetramer of ~240 kDa glycoprotein subunits. IP3R protein is structurally and functionally related to one other important intracellular calcium-release channel, the ryanodine receptor. The similarity between the two receptors continues at the physiological level owing to a physical association each receptor can have with the immunophilin protein, FKBP12. Phosphorylation of the IP3R by PKC causes an increase in IP3-mediated calcium release. Concomitantly, the phosphatase activity of calcineurin is stimulated upon its association with the FKBP12-IP3R complex. Calcium release is reduced when the PKC target site on the IP3R is dephosphorylated by calcineurin resulting in calcium oscillations. Mammalian IP3R subunits are the product of three distinct genes that are widely expressed and differentially regulated. IP3R type I (IP3R-I) has been detected in heart, liver, kidney, ovary, and Purkinje neurons of the cerebellum. IP3R-II is found predominantly in the brain. IP3R-III is known to be expressed in pancreatic islets, kidney, and the gastrointestinal tract.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cilia and flagella associated protein 48; inositol 1,4,5-triphosphate receptor 2; inositol 1,4,5-triphosphate receptor 5; inositol 1,4,5-triphosphate receptor, type 2; Inositol 1,4,5-trisphosphate receptor type 2; Inositol 1,4,5-trisphosphate type V receptor; Inositol 1,4,5-trisphosphate-gated calcium channel ITPR2; inositol triphosphate receptor type 2; inositol trisphosphate receptor type 2; insP3R2; IP3 receptor; IP3 receptor isoform 2; IP3R; IP3R 2; Type 2 inositol 1,4,5-trisphosphate receptor; Type 2 InsP3 receptor
Gene Aliases: AI649341; ANHD; CFAP48; InsP3R-2; InsP3R-5; INSP3R2; IP3R2; ITPR2; Itpr5
UniProt ID: (Human) Q14571, (Mouse) Q9Z329, (Rat) P29995
Entrez Gene ID: (Human) 3709, (Mouse) 16439, (Rat) 81678
inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity
calcium ion transmembrane transporter activity
phosphatidylinositol binding
ion channel activity
calcium channel activity
calcium ion binding
calcium-release channel activity
inositol 1,4,5 trisphosphate binding
ligand-gated ion channel
response to hypoxia
transport
signal transduction
platelet activation
inositol phosphate-mediated signaling
regulation of insulin secretion
release of sequestered calcium ion into cytosol
cellular response to cAMP
cellular response to ethanol
regulation of cardiac conduction
ion transport
calcium ion transport
transmembrane transport
calcium ion transmembrane transport
cellular response to hypoxia
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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