PA1-904 detects inositol 1,4,5-trisphosphate receptor type-II (IP3R-II) from rat and mouse tissues.
PA1-904 has been successfully used in immunohistochemistry, immunofluorescence and immunocytochemistry procedures. Immunohistochemical staining of IP3R-II in rat brain with PA1-904 results in staining of the hippocampus, corpus collosum, and cerebellum. This product fails to detect IP3R-II in Western blot procedures.
PA1-904 immunizing peptide corresponds to amino acid residues 317-334 from rat IP3R-II. PA1-904 immunizing peptide (Cat. # PEP-024) is available for use in neutralization and control experiments.
Inositol 1,4,5-trisphosphate (IP3) is a second messenger for many growth factors, hormones, and neuro-transmitters. Upon binding to IP3R, IP3 triggers the release of intracellular, luminal calcium to the cytosol. Functional IP3R is a homo- or heterotetramer of ~240 kDa glycoprotein subunits. IP3R protein is structurally and functionally related to one other important intracellular calcium-release channel, the ryanodine receptor. The similarity between the two receptors continues at the physiological level owing to a physical association each receptor can have with the immunophilin protein, FKBP12. Phosphorylation of the IP3R by PKC causes an increase in IP3-mediated calcium release. Concomitantly, the phosphatase activity of calcineurin is stimulated upon its association with the FKBP12-IP3R complex. Calcium release is reduced when the PKC target site on the IP3R is dephosphorylated by calcineurin resulting in calcium oscillations. Mammalian IP3R subunits are the product of three distinct genes that are widely expressed and differentially regulated. IP3R type I (IP3R-I) has been detected in heart, liver, kidney, ovary, and Purkinje neurons of the cerebellum. IP3R-II is found predominantly in the brain. IP3R-III is known to be expressed in pancreatic islets, kidney, and the gastrointestinal tract.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: cilia and flagella associated protein 48; inositol 1,4,5-triphosphate receptor 2; inositol 1,4,5-triphosphate receptor 5; inositol 1,4,5-triphosphate receptor, type 2; Inositol 1,4,5-trisphosphate receptor type 2; Inositol 1,4,5-trisphosphate type V receptor; inositol triphosphate receptor type 2; inositol trisphosphate receptor type 2; insP3R2; IP3 receptor; IP3 receptor isoform 2; IP3R 2; Type 2 inositol 1,4,5-trisphosphate receptor; Type 2 InsP3 receptor
Gene Aliases: AI649341; ANHD; CFAP48; InsP3R-2; InsP3R-5; INSP3R2; IP3R2; ITPR2; Itpr5