Immunofluorescence analysis of IP3 Receptor I was done on 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with IP3 Receptor I Rabbit Polyclonal Antibody (PA1901) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Dog, Human, Mouse, Rat|
|Published species reactivity||Rat, Hamster, Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: N(1829) K K K D D E V D R D A P S R K K A K E(1848)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:250|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-901 detects inositol 1,4,5-trisphosphate receptor type-I (IP3R-I) from canine, mouse and rat tissues.
PA1-901 has been successfully used in Western blot, immunohistochemistry, immunofluoresence and immunoprecipitation procedures. By Western blot, this antibody detects an ~240 kDa protein representing IP3R-I from rat and mouse brain extract. Immunohistochemical staining of IP3R-I in rat cerebellum with PA1-901 results in staining of Purkinje cells.
PA1-901 immunizing peptide corresponds to amino acid residues 1829-1848 from human IP3R-I. This sequence is completely conserved in human, mouse, and rat IP3R-I. PA1-901 immunizing peptide (Cat. # PEP-019) is available for use in neutralization and control experiments.
Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate. Involved in the regulation of epithelial secretion of electrolytes and fluid through the interaction with AHCYL1 (By similarity). Plays a role in ER stress-induced apoptosis. Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CaM kinase II, eventually leading to the activation of downstream apoptosis pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
An optimized surgical approach for obtaining stable extracellular single-unit recordings from the cerebellum of head-fixed behaving mice.
PA1-901 was used in immunohistochemistry - paraffin section to discuss potential technical problems while performing vivo electrophysiological recordings
|White JJ,Lin T,Brown AM,Arancillo M,Lackey EP,Stay TL,Sillitoe RV||Journal of neuroscience methods (262:21)||2016|
Pumilio1 haploinsufficiency leads to SCA1-like neurodegeneration by increasing wild-type Ataxin1 levels.
PA1-901 was used in immunocytochemistry to elucidate how post-translational modification of ATAXIN1 levels contributes to spinocerebellar ataxia type 1.
|Gennarino VA,Singh RK,White JJ,De Maio A,Han K,Kim JY,Jafar-Nejad P,di Ronza A,Kang H,Sayegh LS,Cooper TA,Orr HT,Sillitoe RV,Zoghbi HY||Cell (160:1087)||2015|
Efficient generation of mature cerebellar Purkinje cells from mouse embryonic stem cells.
PA1-901 was used in immunocytochemistry to investigate the differentiation into cerebellar Purkinje cells from mouse embryonic stem cells
|Tao O,Shimazaki T,Okada Y,Naka H,Kohda K,Yuzaki M,Mizusawa H,Okano H||Journal of neuroscience research (88:234)||2010|
Multiple receptor interactions trigger release of membrane and intracellular calcium stores critical for herpes simplex virus entry.
PA1-901 was used in immunocytochemistry to study the role of calcium in herpes simplex viruses entry.
|Cheshenko N,Liu W,Satlin LM,Herold BC||Molecular biology of the cell (18:3119)||2007|
Plasma and intracellular membrane inositol 1,4,5-trisphosphate receptors mediate the Ca(2+) increase associated with the ATP-induced increase in ciliary beat frequency.
PA1-901 was used in immunocytochemistry to study the regulation of ciliary beat frequency
|Barrera NP,Morales B,Villalón M||American journal of physiology. Cell physiology (287:C1114)||2004|
IP3 receptors and associated Ca2+ signals localize to satellite cells and to components of the neuromuscular junction in skeletal muscle.
PA1-901 was used in immunocytochemistry to demonstrate the effect of electrical activity on IP3-associated calcium signals.
|Powell JA,Molgó J,Adams DS,Colasante C,Williams A,Bohlen M,Jaimovich E||The Journal of neuroscience : the official journal of the Society for Neuroscience (23:8185)||2003|
Immunocytochemical evidence for co-expression of Type III IP3 receptor with signaling components of bitter taste transduction.
PA1-901 was used in immunocytochemistry to study the role of type III IP3 receptor (IP3R3) in bitter taste transduction
|Clapp TR,Stone LM,Margolskee RF,Kinnamon SC||BMC neuroscience (2:null)||2003|
|Not Applicable||Not Cited||
Chronic enrichment of hepatic endoplasmic reticulum-mitochondria contact leads to mitochondrial dysfunction in obesity.
PA1-901 was used in western blot to study how obesity alters mitochondria-associated ER membranes
|Arruda AP,Pers BM,Parlakgül G,Güney E,Inouye K,Hotamisligil GS||Nature medicine (20:1427)||2014|
TGFß1 downregulates neurite outgrowth, expression of Ca2+ transporters, and mitochondrial dynamics of in vitro cerebellar granule cells.
PA1-901 was used in western blot to study changes in cerebellar granular cell neurite outgrowth, calcium transporters, and mitochondrial biology in response to TGF-beta1
|Jaskova K,Pavlovicova M,Cagalinec M,Lacinova L,Jurkovicova D||Neuroreport (25:340)||2014|
Triadin/Junctin double null mouse reveals a differential role for Triadin and Junctin in anchoring CASQ to the jSR and regulating Ca(2+) homeostasis.
PA1-901 was used in western blot to study the roles of triadin and junctin in anchoring calsequestrin to the junctional sarcoplasmic reticulum and in Ca(2+) homeostasis using triadin/junctin (-/-) knockout mice
|Boncompagni S,Thomas M,Lopez JR,Allen PD,Yuan Q,Kranias EG,Franzini-Armstrong C,Perez CF||PloS one (7:null)||2012|
Increased arterial smooth muscle Ca2+ signaling, vasoconstriction, and myogenic reactivity in Milan hypertensive rats.
PA1-901 was used in western blot to investigate the arterial smooth muscle calcium signaling and vascular functions in Milan hypertensive rats
|Linde CI,Karashima E,Raina H,Zulian A,Wier WG,Hamlyn JM,Ferrari P,Blaustein MP,Golovina VA||American journal of physiology. Heart and circulatory physiology (302:H611)||2012|
Ablation of skeletal muscle triadin impairs FKBP12/RyR1 channel interactions essential for maintaining resting cytoplasmic Ca2+.
PA1-901 was used in western blot to investigate the importance of triadins in the regulation of resting cytoplasmic calcium
|Eltit JM,Feng W,Lopez JR,Padilla IT,Pessah IN,Molinski TF,Fruen BR,Allen PD,Perez CF||The Journal of biological chemistry (285:38453)||2010|
Paclitaxel accelerates spontaneous calcium oscillations in cardiomyocytes by interacting with NCS-1 and the InsP3R.
PA1-901 was used in western blot to investigate the effect of taxol on calcium signaling and calcium oscillation
|Zhang K,Heidrich FM,DeGray B,Boehmerle W,Ehrlich BE||Journal of molecular and cellular cardiology (49:829)||2010|
A novel postsynaptic mechanism for heterosynaptic sharing of short-term plasticity.
PA1-901 was used in western blot to evaluate the role of calcium signalling in heterosynaptic plasticity
|Reissner KJ,Pu L,Schaffhausen JH,Boyle HD,Smith IF,Parker I,Carew TJ||The Journal of neuroscience : the official journal of the Society for Neuroscience (30:8797)||2010|
Inhibition of type I and III IP(3)Rs by TGF-beta is associated with impaired calcium release in mesangial cells.
PA1-901 was used in western blot to study the mechanism for the inhibitory effect of TGF-beta on inositol 1,4,5-trisphosphate receptors in mesangial cells.
|Sharma K,Mc Gowan TA,Wang L,Madesh M,Kaspar V,Szalai G,Thomas AP,Hajnóczky G||American journal of physiology. Renal physiology (278:F1022)||2000|
Individual subtypes of enteroendocrine cells in the mouse small intestine exhibit unique patterns of inositol 1,4,5-trisphosphate receptor expression.
PA1-901 was used in immunohistochemistry to investigate the patterns of inositol 1,4,5-trisphosphate receptor expression in individual subtypes of enteroendocrine cells in the mouse small intestine
|Wang S,Liu J,Li L,Wice BM||The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (52:53)||2004|
ET-1 activates Ca2+ sparks in PASMC: local Ca2+ signaling between inositol trisphosphate and ryanodine receptors.
PA1-901 was used in immunohistochemistry to suggest that ET-1 activation of calcium sparks is mediated via the ETA receptor-PLC-IP3 pathway
|Zhang WM,Yip KP,Lin MJ,Shimoda LA,Li WH,Sham JS||American journal of physiology. Lung cellular and molecular physiology (285:L680)||2003|
Type-specific inositol 1,4,5-trisphosphate receptor localization in the vomeronasal organ and its interaction with a transient receptor potential channel, TRPC2.
PA1-901 was used in immunohistochemistry to investigate the localization and role of inositol 1,4,5-trisphosphate receptor in the vomeronasal organ
|Brann JH,Dennis JC,Morrison EE,Fadool DA||Journal of neurochemistry (83:1452)||2002|