|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues N(1829) K K K D D E V D R D A P S R K K A K E(1848) of human IP3 Receptor Type I.|
|Storage buffer||whole serum diluted in PBS|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA3-901A detects inositol 1,4,5-triphosphate receptor type-I (IP3R-I) from mouse, human and rat tissues.
PA3-901A has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~240-260 kDa protein representing IP3R-I from rat and mouse brain extract. PA3-901A is a remanufactured lot of PA3-901 which has previously been used in Western blot, immunoprecipitation and immunohistochemistry procedures.
The PA3-901A immunogen is a synthetic peptide corresponding to residues N(1829) K K K D D E V D R D A P S R K K A K E(1848) of human IP3 Receptor Type I. This sequence is 100% conserved in human, mouse, and rat IP3R-I protein. This peptide (Cat. # PEP-019) is available for use in neutralization and control experiments.
Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate. Involved in the regulation of epithelial secretion of electrolytes and fluid through the interaction with AHCYL1 (By similarity). Plays a role in ER stress-induced apoptosis. Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CaM kinase II, eventually leading to the activation of downstream apoptosis pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Presenilin-2 dampens intracellular Ca2+ stores by increasing Ca2+ leakage and reducing Ca2+ uptake.
PA3-901A was used in western blot to investigate the mechanism for the regulation of calcium concentration of intracellular stores mediated by presenilin-2
|Brunello L,Zampese E,Florean C,Pozzan T,Pizzo P,Fasolato C||Journal of cellular and molecular medicine (13:3358)||2009|
Thrombin downregulates muscle acetylcholine receptors via an IP3 signaling pathway by activating its G-protein-coupled protease-activated receptor-1.
PA3-901A was used in immunocytochemistry to investigate the mechanism for the effect of thrombin on muscle acetylcholine receptors
|Faraut B,Barbier J,Ravel-Chapuis A,Doyennette MA,Jandrot-Perrus M,Verdière-Sahuqué M,Schaeffer L,Koenig J,Hantaï D||Journal of cellular physiology (196:105)||2003|