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Product Details
PA5-19917
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
2 µg/mL | - |
Immunohistochemistry (IHC) |
20 µg/mL | - |
Immunocytochemistry (ICC/IF) |
2 µg/mL | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
A peptide corresponding to amino acids near the carboxy terminus of human IRAK2. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS |
Contains |
0.02% sodium azide |
Storage conditions |
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C |
RRID |
AB_11153923 |
Product Specific Information
In Western blot applications, this antibody detects a band at ~65kDa. A suggested positive control is Hela cell lysate.
PA5-19917 can be used with blocking peptide PEP-0043.
Target Information
IRAK2 (interleukin-1 receptor-associated kinase-like 2) binds to the IL-1 type I receptor following IL-1 engagment and triggers intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kappa-B, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2. Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kappa-B activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kappa-B activation. Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory responses to cytokine IL-1 and LPS. IRAK2 is expressed in a variety of human tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: interleukin-1 receptor associated kinase-2; interleukin-1 receptor-associated kinase 2; Interleukin-1 receptor-associated kinase-like 2; IRAK-2; MGC150550
Gene Aliases: IRAK-2; IRAK2
UniProt ID: (Human) O43187
Entrez Gene ID: (Human) 3656
activation of MAPK activity
regulation of cytokine-mediated signaling pathway
toll-like receptor signaling pathway
MyD88-dependent toll-like receptor signaling pathway
protein phosphorylation
inflammatory response
I-kappaB kinase/NF-kappaB signaling
JNK cascade
lipopolysaccharide-mediated signaling pathway
negative regulation of NF-kappaB transcription factor activity
toll-like receptor 9 signaling pathway
positive regulation of NF-kappaB transcription factor activity
nucleotide-binding oligomerization domain containing signaling pathway
interleukin-1-mediated signaling pathway
response to interleukin-1
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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