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Invitrogen
IRAK4 Polyclonal Antibody
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FIGURE: 1 / 2
IRAK4 Antibody (720328) in ICC/IF
For immunofluorescence analysis, THP-1 cells were fixed and permeabilized for detection of endogenous IRAK4 using Anti- IRAK4 Rabbit Polyclonal Antibody (Product # 720328, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of IRAK4 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c... View More
Product Details
720328
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptides corresponding to Human IRAK4 (aa 40- 56, 404-422)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 30% glycerol, 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.
This antibody is predicted to react with Monkey, Horse and Bat
Target Information
IL-1 receptor associated kinases (IRAKs) are serine/threonine kinases involved in signaling via Toll/IL-1 pathways. Overexpression of IRAK-4 activates NF-kappa-B and MAPK pathways. IRAK-4 interacts with and phosphorylates IRAK-1. IRAK-4-deficient animals are completely resistant to the challenge with LPS. Animals and humans lacking IRAK-4 are impaired in their responses to viral and bacterial challenges. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses and in innate immunity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: interleukin-1; Interleukin-1 receptor-associated kinase 4; IRAK-4; IRAK-4 mutated form 1; IRAK-4 mutated form 2; IRAK4; protein kinase; IRAK4S; protein kinase; protein kinase; Renal carcinoma antigen NY-REN-64; unnamed protein product
Gene Aliases: IMD67; IPD1; IRAK-4; IRAK4; NY-REN-64; REN64
UniProt ID: (Human) Q9NWZ3
Entrez Gene ID: (Human) 51135
toll-like receptor signaling pathway
immune system process
neutrophil mediated immunity
MyD88-dependent toll-like receptor signaling pathway
signal transduction
JNK cascade
Toll signaling pathway
cytokine-mediated signaling pathway
positive regulation of defense response
lipopolysaccharide-mediated signaling pathway
toll-like receptor 4 signaling pathway
toll-like receptor 9 signaling pathway
intracellular signal transduction
NIK/NF-kappaB signaling
interleukin-33-mediated signaling pathway
positive regulation of I-kappaB kinase/NF-kappaB signaling
innate immune response
positive regulation of smooth muscle cell proliferation
interleukin-1-mediated signaling pathway
neutrophil migration
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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