|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide between amino acids 700-800 of the human IRE1 alpha protein.[Swiss-Prot#075460]|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris glycine with 0.15M NaCl|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
This antibody does not detect monkey IRE1 alpha.
Suggested positive control: Pancreatic islets and insulinoma cells.
IRE1 acts as the sensor of unfolded proteins in the ER that initiates transmittance of the unfolded protein signal from the ER to the nucleus by splicing XBP1 mRNA converting it into a potent unfolded-protein response transcriptional activator. It is a transmembrane protein that has both serine-threonine kinase and endoribonuclease activities. Null mutants are viable, but IRE1 is essential for viablility under stress conditions that cause unfolded proteins to accumulate in the ER.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Attenuation of unfolded protein response and apoptosis by mReg2 induced GRP78 in mouse insulinoma cells.
PA1-16928 was used in western blot to study the ability of mReg2-induced expression of GPR78 to protect murine insulinoma cells against endoplasmic reticulum stress
|Liu L,Chowdhury S,Fang X,Liu JL,Srikant CB||FEBS letters (588:2016)||2014|