Invitrogen

IREB2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

View all (8) IREB2 antibodies

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IREB2 Antibody in Western Blot (WB) — Western blot was performed using Anti-IREB2 Polyclonal Antibody (Product # PA5-41782) and a 105 kDa band corresponding to IREB2 was observed only in Mouse, Rat Kidney; Mouse, Rat Liver and Mouse Testis when compared to other tissues which are reported to be negative. Tissue extracts of Mouse Kidney (Lane 1), Rat Kidney (Lane 2), Mouse Liver (Lane 3), Rat Liver (Lane 4), Mouse skeletal Muscle (Lane 5), Rat skeletal Muscle (Lane 6), Mouse Heart (Lane 7), Rat Heart (Lane 8), Mouse Brown Adipose (Lane 9) and Mouse Testis (Lane 10) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0342BOX). Antibody were Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). (Ref :DOI: 10.1371/journal.pone.0098072, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1271751/).

IREB2 Antibody in Relative expression — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of IREB2 was observed in Mouse Kidney, Rat Kidney, Mouse Liver, Rat Liver in higher levels in comparison to low levels in Mouse skeletal Muscle, Rat skeletal Muscle, Mouse Heart, Rat heart and Mouse Brown Adipose using Anti-IREB2 Polyclonal Antibody (Product # PA5-41782) in Western Blot. (Ref :DOI: 10.1371/journal.pone.0098072, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1271751/). {RE}

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

Product Specifications

Species Reactivity

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide directed towards the middle region of human IREB2

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS with 2% sucrose

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2608705

Product Specific Information

Peptide sequence: IQINLNSIVP SVSGPKRPQD RVAVTDMKSD FQACLNEKVG FKGFQIAAEK

Sequence homology: Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 93%; Pig: 100%; Rabbit: 93%; Rat: 100%; Zebrafish: 93%

Target Information

Iron regulatory protein 2 (IRP2) is a central post-transcriptional regulator of cellular and systemic iron metabolism. It is a cytosolic RNA-binding protein that binds to stem-loop structures. IRP2 is regulated by iron-mediated proteolysis. Hypoxia is important for normal tissue physiology as well as being a component of several pathophysiological conditions, including heart and cerebrovascular diseases and tumor growth. Because iron and oxygen are closely related, IRP2 also plays a role during hypoxia (low oxygen).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FLJ23381; IRE-BP 2; Iron regulatory protein 2; iron-responsive element binding protein 2; Iron-responsive element-binding protein 2; IRP2

Gene Aliases: ACO3; D9Ertd85e; IREB2; IRP2; IRP2AD

UniProt ID: (Human) P48200, (Mouse) Q811J3

Entrez Gene ID: (Human) 3658, (Mouse) 64602

Molecular Function: dehydratase hydratase lyase