Invitrogen

IREB2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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IREB2 Antibody in Western Blot (WB) — Western blot was performed using Anti-IREB2 Polyclonal Antibody (Product # PA5-41782) and a 105 kDa band corresponding to IREB2 was observed only in Mouse, Rat Kidney; Mouse, Rat Liver and Mouse Testis when compared to other tissues which are reported to be negative. Tissue extracts of Mouse Kidney (Lane 1), Rat Kidney (Lane 2), Mouse Liver (Lane 3), Rat Liver (Lane 4), Mouse skeletal Muscle (Lane 5), Rat skeletal Muscle (Lane 6), Mouse Heart (Lane 7), Rat Heart (Lane 8), Mouse Brown Adipose (Lane 9) and Mouse Testis (Lane 10) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0342BOX). Antibody were Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). (Ref :DOI: 10.1371/journal.pone.0098072, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1271751/).

IREB2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of IREB2 was observed in Mouse Kidney, Rat Kidney, Mouse Liver, Rat Liver in higher levels in comparison to low levels in Mouse skeletal Muscle, Rat skeletal Muscle, Mouse Heart, Rat heart and Mouse Brown Adipose using Anti-IREB2 Polyclonal Antibody (Product # PA5-41782) in Western Blot. (Ref :DOI: 10.1371/journal.pone.0098072, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1271751/). {RE}

Product Details

PA5-41782

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide directed towards the middle region of human IREB2
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS with 2% sucrose
Contains	0.09% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2608705

Product Specific Information

Peptide sequence: IQINLNSIVP SVSGPKRPQD RVAVTDMKSD FQACLNEKVG FKGFQIAAEK

Sequence homology: Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 93%; Pig: 100%; Rabbit: 93%; Rat: 100%; Zebrafish: 93%

Target Information

Iron regulatory protein 2 (IRP2) is a central post-transcriptional regulator of cellular and systemic iron metabolism. It is a cytosolic RNA-binding protein that binds to stem-loop structures. IRP2 is regulated by iron-mediated proteolysis. Hypoxia is important for normal tissue physiology as well as being a component of several pathophysiological conditions, including heart and cerebrovascular diseases and tumor growth. Because iron and oxygen are closely related, IRP2 also plays a role during hypoxia (low oxygen).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FLJ23381; IRE-BP 2; Iron regulatory protein 2; iron-responsive element binding protein 2; Iron-responsive element-binding protein 2; IRP2

Gene Aliases: ACO3; D9Ertd85e; IREB2; IRP2; IRP2AD

UniProt ID: (Human) P48200, (Mouse) E9QPM2

Entrez Gene ID: (Human) 3658, (Mouse) 64602

Molecular Function: RNA metabolism protein