Invitrogen

IREB2 Polyclonal Antibody

Product Image Gallery

IREB2 Antibody in Immunocytochemistry (ICC) — Immunocytochemical analysis of IREB2 in HepG2 cells using a IREB2 Polyclonal antibody (Product # PA5-86058) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

IREB2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of IREB2 of paraffin-embedded Human placenta tissue using a IREB2 Polyclonal antibody (Product #PA5-86058). Counter stained with hematoxylin.

IREB2 Antibody in Western Blot (WB) — Western blot analysis of IREB2 in different lysates using a Polyclonal antibody (Product #PA5-86058) at a dilution of 1:500. Positive control: Lane1: K562, Lane 2: Mouse kidney tissue, Lane 3: Rat skin tissue.

IREB2 Antibody in Flow Cytometry (Flow) — Flow Cytometric analysis of IREB2 in K562 cells using a IREB2 Polyclonal Antibody (Product # PA5-86058) at a dilution of 1:100, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black).

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1:50-1:100

Immunocytochemistry (ICC)

1:50-1:200

Immunofluorescence (IF)

1:50-1:200

Immunohistochemistry (IHC)

1:50-1:200

Western Blot (WB)

1:500-1:1000

Product Specifications

Species

Human, Mouse, Rat

Expression System

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein within human IREB2 aa 27-301

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 50% Glycerol, 0.2% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2802859

Target Information

Iron regulatory protein 2 (IRP2) is a central post-transcriptional regulator of cellular and systemic iron metabolism. It is a cytosolic RNA-binding protein that binds to stem-loop structures. IRP2 is regulated by iron-mediated proteolysis. Hypoxia is important for normal tissue physiology as well as being a component of several pathophysiological conditions, including heart and cerebrovascular diseases and tumor growth. Because iron and oxygen are closely related, IRP2 also plays a role during hypoxia (low oxygen).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FLJ23381; IRE-BP 2; Iron regulatory protein 2; iron-regulatory protein 2; iron-responsive element binding protein 2; Iron-responsive element-binding protein 2; IRP2

Gene Aliases: ACO3; D9Ertd85e; IREB2; IRP2; IRP2AD

UniProt ID: (Human) P48200, (Rat) Q62751, (Mouse) Q811J3

Entrez Gene ID: (Human) 3658, (Rat) 64831, (Mouse) 64602

Molecular Function: dehydratase hydratase lyase