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IRF5 Polyclonal Antibody

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IRF5 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells using 10547-1-AP (IRF5 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

IRF5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 10547-1-AP (IRF5 antibody) at dilution of 1:50 (under 10x lens).

IRF5 Antibody in Western Blot (WB) — Human heart tissue were subjected to SDS PAGE followed by western blot with 10547-1-AP (IRF5 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

IRF5 Antibody in Flow Cytometry (Flow) — 1X10^6 HepG2 cells were stained with 0.2ug IRF5 antibody (10547-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

IRF5 Antibody in Immunoprecipitation (IP) — IP result of anti-IRF5 (IP:10547-1-AP, 4 ug; Detection:10547-1-AP 1:300) with HeLa cells lysate 1600 ug.

Product Details

10547-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 11 publications 11 publications
Immunohistochemistry (IHC)	-	View 5 publications 5 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:10-1:100	View 2 publications 2 publications
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat, Sheep
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	IRF5 Fusion Protein Ag0841 (172-498 aa encoded by BC004201) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.17 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

10547-1-AP is a rabbit polyclonal antibody raised against the C-terminal of human IRF5.

Immunogen sequence: PTLRPPTLQ PPTLQPPVVL GPPAPDPSPL APPPGNPAGF RELLSEVLEP GPLPASLPPA GEQLLPDLLI SPHMLPLTDL EIKFQYRGRP PRALTISNPH GCRLFYSQLE ATQEQVELFG PISLEQVRFP SPEDIPSDKQ RFYTNQLLDV LDRGLILQLQ GQDLYAIRLC QCKVFWSGPC ASAHDSCPNP IQREVKTKLF SLEHFLNELI LFQKGQTNTP PPFEIFFCFG EEWPDRKPRE KKLITVQVVP VAARLLLEMF SGELSWSADS IRLQISNPDL KDRMVEQFKE LHHIWQSQQR LQPVAQAPPG AGLGVGQGPW PMHPAGMQ (172-498 aa encoded by BC004201)

Target Information

This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative splice variants encoding different isoforms exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Quercetin ameliorates kidney injury and fibrosis by modulating M1/M2 macrophage polarization.

Biochemical pharmacology

Lu H,Wu L,Liu L,Ruan Q,Zhang X,Hong W,Wu S,Jin G,Bai Y

Wed Aug 01 00:00:00 EDT 2018

Quercetin ameliorates kidney injury and fibrosis by modulating M1/M2 macrophage polarization.

Biochemical pharmacology

Lu H,Wu L,Liu L,Ruan Q,Zhang X,Hong W,Wu S,Jin G,Bai Y

Wed Aug 01 00:00:00 EDT 2018

Capsular Polysaccharide is a Main Component of Mycoplasma ovipneumoniae in the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells.

Mediators of inflammation

Jiang Z,Song F,Li Y,Xue D,Deng G,Li M,Liu X,Wang Y

Tue Mar 27 00:00:00 EDT 2018

Dual role of YM1+ M2 macrophages in allergic lung inflammation.

Scientific reports

Draijer C,Robbe P,Boorsma CE,Hylkema MN,Melgert BN

Fri Mar 23 00:00:00 EDT 2018

Chronic inflammation is a feature of Achilles tendinopathy and rupture.

British journal of sports medicine

Dakin SG,Newton J,Martinez FO,Hedley R,Gwilym S,Jones N,Reid HAB,Wood S,Wells G,Appleton L,Wheway K,Watkins B,Carr AJ

Thu Mar 01 00:00:00 EST 2018

Tumor Suppressor p53 Stimulates the Expression of Epstein-Barr Virus Latent Membrane Protein 1.

Journal of virology

Wang Q,Lingel A,Geiser V,Kwapnoski Z,Zhang L

Sun Oct 15 00:00:00 EDT 2017

Specific detection of interferon regulatory factor 5 (IRF5): A case of antibody inequality.

Scientific reports

Li D,De S,Li D,Song S,Matta B,Barnes BJ

Tue Aug 02 00:00:00 EDT 2016

Distinct macrophage phenotypes in allergic and nonallergic lung inflammation.

American journal of physiology. Lung cellular and molecular physiology

Robbe P,Draijer C,Borg TR,Luinge M,Timens W,Wouters IM,Melgert BN,Hylkema MN

Sun Feb 15 00:00:00 EST 2015

Hepatic Localization of Macrophage Phenotypes during Fibrogenesis and Resolution of Fibrosis in Mice and Humans.

Frontiers in immunology

Beljaars L,Schippers M,Reker-Smit C,Martinez FO,Helming L,Poelstra K,Melgert BN

Wed Sep 24 00:00:00 EDT 2014

Hepatic Localization of Macrophage Phenotypes during Fibrogenesis and Resolution of Fibrosis in Mice and Humans.

Frontiers in immunology

Beljaars L,Schippers M,Reker-Smit C,Martinez FO,Helming L,Poelstra K,Melgert BN

Wed Sep 24 00:00:00 EDT 2014

Characterization of transfusion-elicited acute antibody-mediated rejection in a rat model of kidney transplantation.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Huang G,Wilson NA,Reese SR,Jacobson LM,Zhong W,Djamali A

Thu May 01 00:00:00 EDT 2014

4F decreases IRF5 expression and activation in hearts of tight skin mice.

PloS one

Xu H,Krolikowski JG,Jones DW,Ge ZD,Pagel PS,Pritchard KA,Weihrauch D

Thu Sep 05 00:00:00 EDT 2013

Characterization of macrophage phenotypes in three murine models of house-dust-mite-induced asthma.

Mediators of inflammation

Draijer C,Robbe P,Boorsma CE,Hylkema MN,Melgert BN

Mon Aug 19 00:00:00 EDT 2013

A study on the immunomodulation of polysaccharopeptide through the TLR4-TIRAP/MAL-MyD88 signaling pathway in PBMCs from breast cancer patients.

Immunopharmacology and immunotoxicology

Wang J,Dong B,Tan Y,Yu S,Bao YX

Thu Aug 01 00:00:00 EDT 2013

Interleukin-4-induced β-catenin regulates the conversion of macrophages to multinucleated giant cells.

Molecular immunology

Binder F,Hayakawa M,Choo MK,Sano Y,Park JM

Sat Jun 01 00:00:00 EDT 2013

PPE38 of Mycobacterium marinum triggers the cross-talk of multiple pathways involved in the host response, as revealed by subcellular quantitative proteomics.

Journal of proteome research

Wang H,Dong D,Tang S,Chen X,Gao Q

Fri May 03 00:00:00 EDT 2013

PEGylation of interleukin-10 improves the pharmacokinetic profile and enhances the antifibrotic effectivity in CCl₄-induced fibrogenesis in mice.

Journal of controlled release : official journal of the Controlled Release Society

Mattos A,de Jager-Krikken A,de Haan M,Beljaars L,Poelstra K

Mon Aug 20 00:00:00 EDT 2012

Activation of interferon regulatory factor 5 by site specific phosphorylation.

PloS one

Chang Foreman HC,Van Scoy S,Cheng TF,Reich NC

Thu Aug 02 00:00:00 EDT 2012

Activation of interferon regulatory factor 5 by site specific phosphorylation.

PloS one

Chang Foreman HC,Van Scoy S,Cheng TF,Reich NC

Thu Aug 02 00:00:00 EDT 2012

Smoking during pregnancy influences the maternal immune response in mice and humans.

American journal of obstetrics and gynecology

Prins JR,Hylkema MN,Erwich JJ,Huitema S,Dekkema GJ,Dijkstra FE,Faas MM,Melgert BN

Sun Jul 01 00:00:00 EDT 2012

Interferon regulatory factor 4 (IRF-4) targets IRF-5 to regulate Epstein-Barr virus transformation.

The Journal of biological chemistry

Xu D,Meyer F,Ehlers E,Blasnitz L,Zhang L

Fri May 20 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: Interferon regulatory factor 5; interferon regulatory factor 5 bone marrow; IRF-5; OTTHUMP00000212907

Gene Aliases: AW491843; IRF5; mirf5; SLEB10

UniProt ID: (Human) Q13568, (Mouse) P56477

Entrez Gene ID: (Human) 3663, (Rat) 296953, (Mouse) 27056

Molecular Function: winged helix/forkhead transcription factor

Function(s)

transcription, DNA-templated response to peptidoglycan response to muramyl dipeptide positive regulation of interferon-alpha production positive regulation of interferon-beta production positive regulation of interleukin-12 production positive regulation of apoptotic process positive regulation of transcription from RNA polymerase II promoter defense response to virus interferon-gamma-mediated signaling pathway type I interferon signaling pathway cytokine-mediated signaling pathway immune system process regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter innate immune response

Process(es)

regulatory region DNA binding transcription factor activity, sequence-specific DNA binding protein binding DNA binding

IRF5 Polyclonal Antibody

Product Details

10547-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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