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IRF5 Polyclonal Antibody

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Antibody Testing Data (17)

IRF5 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 17

IRF5 Antibody (10547-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells using 10547-1-AP (IRF5 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Product Image Gallery

IRF5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue slide using (Product # 10547-1-AP) IRF5 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IRF5 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with (Product # 10547-1-AP) IRF5 antibody at dilution of 1:5,000 incubated at room temperature for 1.5 hours.

IRF5 Antibody in Flow Cytometry (Flow) — 1X10^6 HepG2 cells were stained with 0.2ug IRF5 antibody (10547-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

IRF5 Antibody in Immunoprecipitation (IP) — IP result of anti-IRF5 (IP:10547-1-AP, 4 ug; Detection:10547-1-AP 1:300) with HeLa cells lysate 1600 ug.

Product Details

10547-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 21 publications 21 publications

Immunohistochemistry (IHC)

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 4 publications 4 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat, Sheep

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

IRF5 Fusion Protein Ag0841 (172-498 aa encoded by BC004201)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

10547-1-AP is a rabbit polyclonal antibody raised against the C-terminal of human IRF5.

Immunogen sequence: PTLRPPTLQ PPTLQPPVVL GPPAPDPSPL APPPGNPAGF RELLSEVLEP GPLPASLPPA GEQLLPDLLI SPHMLPLTDL EIKFQYRGRP PRALTISNPH GCRLFYSQLE ATQEQVELFG PISLEQVRFP SPEDIPSDKQ RFYTNQLLDV LDRGLILQLQ GQDLYAIRLC QCKVFWSGPC ASAHDSCPNP IQREVKTKLF SLEHFLNELI LFQKGQTNTP PPFEIFFCFG EEWPDRKPRE KKLITVQVVP VAARLLLEMF SGELSWSADS IRLQISNPDL KDRMVEQFKE LHHIWQSQQR LQPVAQAPPG AGLGVGQGPW PMHPAGMQ (172-498 aa encoded by BC004201)

Target Information

This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative splice variants encoding different isoforms exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Early macrophage response to obesity encompasses Interferon Regulatory Factor 5 regulated mitochondrial architecture remodelling.

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Early macrophage response to obesity encompasses Interferon Regulatory Factor 5 regulated mitochondrial architecture remodelling.

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Acupuncture relieves the visceral pain of diarrhea-predominant irritable bowel syndrome rats by regulating P2X4 expression.

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Sedum sarmentosum Bunge extract alleviates inflammation and kidney injury via inhibition of M1-macrophage polarization.

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Sedum sarmentosum Bunge extract alleviates inflammation and kidney injury via inhibition of M1-macrophage polarization.

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Sun Sep 01 00:00:00 EDT 2019

Sedum sarmentosum Bunge extract alleviates inflammation and kidney injury via inhibition of M1-macrophage polarization.

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Chronic inflammation is a feature of Achilles tendinopathy and rupture.

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Quercetin ameliorates kidney injury and fibrosis by modulating M1/M2 macrophage polarization.

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Hepatic Localization of Macrophage Phenotypes during Fibrogenesis and Resolution of Fibrosis in Mice and Humans.

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Hepatic Localization of Macrophage Phenotypes during Fibrogenesis and Resolution of Fibrosis in Mice and Humans.

Frontiers in immunology

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Wang H,Dong D,Tang S,Chen X,Gao Q

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Characterization of macrophage phenotypes in three murine models of house-dust-mite-induced asthma.

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Draijer C,Robbe P,Boorsma CE,Hylkema MN,Melgert BN

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Characterization of macrophage phenotypes in three murine models of house-dust-mite-induced asthma.

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Smoking during pregnancy influences the maternal immune response in mice and humans.

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Smoking during pregnancy influences the maternal immune response in mice and humans.

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Prins JR,Hylkema MN,Erwich JJ,Huitema S,Dekkema GJ,Dijkstra FE,Faas MM,Melgert BN

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Activation of interferon regulatory factor 5 by site specific phosphorylation.

PloS one

Chang Foreman HC,Van Scoy S,Cheng TF,Reich NC

Thu Aug 02 00:00:00 EDT 2012

Activation of interferon regulatory factor 5 by site specific phosphorylation.

PloS one

Chang Foreman HC,Van Scoy S,Cheng TF,Reich NC

Thu Aug 02 00:00:00 EDT 2012

Activation of interferon regulatory factor 5 by site specific phosphorylation.

PloS one

Chang Foreman HC,Van Scoy S,Cheng TF,Reich NC

Thu Aug 02 00:00:00 EDT 2012

Activation of interferon regulatory factor 5 by site specific phosphorylation.

PloS one

Chang Foreman HC,Van Scoy S,Cheng TF,Reich NC

Thu Aug 02 00:00:00 EDT 2012

4F decreases IRF5 expression and activation in hearts of tight skin mice.

PloS one

Xu H,Krolikowski JG,Jones DW,Ge ZD,Pagel PS,Pritchard KA,Weihrauch D

Thu Sep 05 00:00:00 EDT 2013

4F decreases IRF5 expression and activation in hearts of tight skin mice.

PloS one

Xu H,Krolikowski JG,Jones DW,Ge ZD,Pagel PS,Pritchard KA,Weihrauch D

Thu Sep 05 00:00:00 EDT 2013

Interferon regulatory factor 4 (IRF-4) targets IRF-5 to regulate Epstein-Barr virus transformation.

The Journal of biological chemistry

Xu D,Meyer F,Ehlers E,Blasnitz L,Zhang L

Fri May 20 00:00:00 EDT 2011

Interferon regulatory factor 4 (IRF-4) targets IRF-5 to regulate Epstein-Barr virus transformation.

The Journal of biological chemistry

Xu D,Meyer F,Ehlers E,Blasnitz L,Zhang L

Fri May 20 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: Interferon regulatory factor 5; interferon regulatory factor 5 bone marrow; IRF-5; OTTHUMP00000212907

Gene Aliases: AW491843; IRF5; mirf5; SLEB10

UniProt ID: (Human) Q13568, (Mouse) P56477

Entrez Gene ID: (Human) 3663, (Rat) 296953, (Mouse) 27056

Molecular Function: winged helix/forkhead transcription factor

Function(s)

transcription, DNA-templated response to peptidoglycan response to muramyl dipeptide positive regulation of interferon-alpha production positive regulation of interferon-beta production positive regulation of interleukin-12 production positive regulation of apoptotic process positive regulation of transcription from RNA polymerase II promoter defense response to virus interferon-gamma-mediated signaling pathway type I interferon signaling pathway cytokine-mediated signaling pathway immune system process regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter innate immune response

Process(es)

regulatory region DNA binding transcription factor activity, sequence-specific DNA binding protein binding DNA binding

IRF5 Polyclonal Antibody

IRF5 Antibody (10547-1-AP) in ICC/IF

Product Details

10547-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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