Invitrogen

IRF7 Polyclonal Antibody

View all (23) IRF7 antibodies

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IRF7 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of 4% paraformaldehyde-fixed Raw264.7 cells labeling IRF7 with IRF7 Polyclonal Antibody (Product # PA5-114526) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI staining (blue).

IRF7 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using IRF7 Polyclonal Antibody (Product # PA5-114526) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

IRF7 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human pancreas tissue using IRF7 Polyclonal Antibody (Product # PA5-114526) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

IRF7 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human colon tissue using IRF7 Polyclonal Antibody (Product # PA5-114526) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

IRF7 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human liver tissue using IRF7 Polyclonal Antibody (Product # PA5-114526) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

IRF7 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded rat brain tissue using IRF7 Polyclonal Antibody (Product # PA5-114526) at 5 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

IRF7 Antibody in Western Blot (WB) — Western blot was performed using Anti-IRF7 Polyclonal Antibody (Product # PA5-114526) and a 55 kDa band corresponding to IRF7 was observed across Hela cells on treatment with IFN alpha. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), HeLa IFN alpha treated (500 units/mL for 12 hours) (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:10000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580). An uncharacterized band (*) was observed at ~110 kDa.

IRF7 Antibody in Western Blot (WB) — Western Blot analysis of IRF7 in A549 WT or IRF7 KO cells. Lysates (10 µg) were loaded onto SDS-PAGE and blots were probed with IRF7 Polyclonal Antibody (Product # PA5-114526) diluted to 0.5 µg/mL and anti-beta actin diluted to 1 µg/mL. 1 h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.

IRF7 Antibody in Western Blot (WB) — Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: IRF7 Polyclonal Antibody (Product # PA5-114526) (1 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

IRF7 Antibody in Western Blot (WB) — Western Blot Validation in Human Tissues. Loading: 15 µg of lysates per lane. Antibodies: IRF7 Polyclonal Antibody (Product # PA5-114526) (1 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

IRF7 Antibody in Western Blot (WB) — Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: IRF7 Polyclonal Antibody (Product # PA5-114526) (1 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

IRF7 Antibody in Western Blot (WB) — Western Blot Validation in Rat Tissues. Loading: 15 µg of lysates per lane. Antibodies: IRF7 Polyclonal Antibody (Product # PA5-114526) (1 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Product Details

PA5-114526

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

2-5 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Anti-IRF7 antibody was raised against a peptide corresponding to 17 amino acids near the center of human IRF7. The immunogen is located within amino acids 200-230 of IRF7.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2890457

Product Specific Information

Positive controls that may be used include: A549 Cell Lysate, HeLa Cell Lysate, HepG2 Cell Lysate

Target Information

Interferons (IFNs) are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-a/b genes. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. IRF7 play a major role in the innate immune pathway, interacting with the Toll-like receptor (TLR) adaptor proteins MyD88 and Tirp/TRAM and functioning as an intermediate TLR4 and TLR9 signaling. There are at least four differentially spliced isoforms of IRF7, although their function has not been clearly established.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Interferon regulatory factor 7; interferon regulatory factor-7H; IRF-7

Gene Aliases: IMD39; IRF-7H; IRF7; IRF7A; IRF7B; IRF7C; IRF7H

UniProt ID: (Human) Q92985, (Mouse) P70434

Entrez Gene ID: (Human) 3665, (Mouse) 54123, (Rat) 293624

Molecular Function: winged helix/forkhead transcription factor

negative regulation of transcription from RNA polymerase II promoter regulation of adaptive immune response transcription from RNA polymerase II promoter cellular response to DNA damage stimulus response to virus immunoglobulin mediated immune response establishment of viral latency regulation of type I interferon production positive regulation of type I interferon production interferon-alpha production interferon-beta production positive regulation of interferon-alpha production positive regulation of interferon-beta production regulation of MyD88-dependent toll-like receptor signaling pathway regulation of MyD88-independent toll-like receptor signaling pathway TRIF-dependent toll-like receptor signaling pathway MDA-5 signaling pathway innate immune response type I interferon biosynthetic process regulation of monocyte differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of immune response interferon-gamma-mediated signaling pathway type I interferon signaling pathway positive regulation of type I interferon-mediated signaling pathway negative regulation of macrophage apoptotic process immune system process transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter regulation of innate immune response

Process(es)

RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding protein binding regulatory region DNA binding transcription factor activity, sequence-specific DNA binding