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Invitrogen
IRF8 Polyclonal Antibody
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FIGURE: 1 / 2
IRF8 Antibody (PA5-78013) in WB
Western blot was performed using Anti-IRF8 Polyclonal Antibody(Product # PA5-78013) and a 50kDa band corresponding to IRF8 was observed across cell lines tested except K-562 and T98G which are reported to be negative. A non-specific band around 95kDa was also observed across cell lines. Nuclear enriched extracts (30 µg lysate) of THP-1 (Lane 1), Raji (Lane 2), Ramos (Lane 3), K-562 (Lane 4) and T98G (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a N... View More
Product Details
PA5-78013
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human IRF8. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.45 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: THP-1 nuclear extract
Predicted Reactivity: Mouse (86%), Rat (85%), Pig (84%), Rhesus Monkey (98%), Bovine (86%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
IRF8 (interferon regulatory factor 8) is a transcriptional activator or repressor. It specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes. IRF8 plays a negative regulatory role in cells of the immune system. It is involved in CD8+ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells. This leads to recognition of AICE sequence, an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of the genes. Mutations in the gene can result in immunodeficiencey 32A and 32B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: H-ICSBP; interferon consensus sequence binding protein 1; Interferon consensus sequence-binding protein; Interferon regulatory factor 8; IRF-8
Gene Aliases: H-ICSBP; ICSBP; ICSBP1; IMD32A; IMD32B; IRF-8; IRF8
UniProt ID: (Human) Q02556
Entrez Gene ID: (Human) 3394
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
phagocytosis
immune response
myeloid cell differentiation
positive regulation of interferon-gamma production
positive regulation of interleukin-12 production
defense response to bacterium
defense response to protozoan
negative regulation of growth of symbiont in host
positive regulation of transcription initiation from RNA polymerase II promoter
interferon-gamma-mediated signaling pathway
type I interferon signaling pathway
cellular response to lipopolysaccharide
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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