Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:2,000 | View 6 publications 6 publications |
Immunohistochemistry (IHC) |
- | View 1 publication 1 publication |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:20-1:200 | - |
Immunocytochemistry (ICC/IF) |
1:20-1:200 | - |
Immunoprecipitation (IP) |
0.5-4.0 µg | - |
Product Specifications | |
---|---|
Species Reactivity |
Human |
Published species |
Fish, Human, Mouse, Pig |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
IRS1 Fusion Protein Ag11714 (895-1243 aa encoded by BC053895) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.3 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C |
Immunogen sequence: EYVNIEFGS DQSGYLSGPV AFHSSPSVRC PSQLQPAPRE EETGTEEYMK MDLGPGRRAA WQESTGVEMG RLGPAPPGAA SICRPTRAVP SSRGDYMTMQ MSCPRQSYVD TSPAAPVSYA DMRTGIAAEE VSLPRATMAA ASSSSAASAS PTGPQGAAEL AAHSSLLGGP QGPGGMSAFT RVNLSPNRNQ SAKVIRADPQ GCRRRHSSET FSSTPSATRV GNTVPFGAGA AVGGGGGSSS SSEDVKRHSS ASFENVWLRP GELGGAPKEP AKLCGAAGGL ENGLNYIDLD LVKDFKQCPQ ECTPEPQPPP PPPPHQPLGS GESSSTRRSS EDLSAYASIS FQKQPEDRQ (895-1243 aa encoded by BC053895)
IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Insulin receptor substrate 1; IRS-1
Gene Aliases: HIRS-1; IRS1
UniProt ID: (Human) P35568
Entrez Gene ID: (Human) 3667
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