Proteintech

IRS1 Polyclonal Antibody

IRS1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

IRS1 Antibody (17509-1-AP) in ICC/IF

Immunofluorescent analysis of A549 cells using 17509-1-AP (IRS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

IRS1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

IRS1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

17509-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 44 publications 44 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Dot blot (DB)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Fish, Human, Mouse, Pig

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

IRS1 Fusion Protein Ag11714 (895-1243 aa encoded by BC053895)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: EYVNIEFGS DQSGYLSGPV AFHSSPSVRC PSQLQPAPRE EETGTEEYMK MDLGPGRRAA WQESTGVEMG RLGPAPPGAA SICRPTRAVP SSRGDYMTMQ MSCPRQSYVD TSPAAPVSYA DMRTGIAAEE VSLPRATMAA ASSSSAASAS PTGPQGAAEL AAHSSLLGGP QGPGGMSAFT RVNLSPNRNQ SAKVIRADPQ GCRRRHSSET FSSTPSATRV GNTVPFGAGA AVGGGGGSSS SSEDVKRHSS ASFENVWLRP GELGGAPKEP AKLCGAAGGL ENGLNYIDLD LVKDFKQCPQ ECTPEPQPPP PPPPHQPLGS GESSSTRRSS EDLSAYASIS FQKQPEDRQ (895-1243 aa encoded by BC053895)

Target Information

IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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