Bethyl Laboratories

IRS1 Polyclonal Antibody

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IRS1 Antibody in Immunohistochemistry (IHC) — Detection of human IRS1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-IRS1 (Cat. No. A301-158A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.

IRS1 Antibody in Western Blot (WB) — Detection of human and mouse IRS1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 µg) and mouse NIH3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-IRS1 antibody A301-158A used for WB at 0.04 µg/ml (A) and 1.0 µg/ml (B) and used for IP at 3 µg/mg lysate. IRS1 was also immunoprecipitated by rabbit anti-IRS1 antibody A301-157A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).

Product Details

A301-158A

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000-1:10,000	-
Immunohistochemistry (IHC)	1:1,000-1:5,000	-
Immunoprecipitation (IP)	2-5 µg/mg lysate	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Between 1192 and 1242
Conjugate	Unconjugated
Form	liquid
Concentration	0.20 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains	0.09% sodium azide
Storage conditions	4° C

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Target Information

IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Insulin receptor substrate 1; IRS-1

Gene Aliases: G972R; HIRS-1; IRS-1; IRS1

UniProt ID: (Human) P35568, (Mouse) P35569

Entrez Gene ID: (Human) 3667, (Mouse) 16367

MAPK cascade signal transduction positive regulation of cell proliferation insulin receptor signaling pathway positive regulation of glucose metabolic process phosphatidylinositol 3-kinase signaling regulation of phosphatidylinositol 3-kinase signaling positive regulation of fatty acid beta-oxidation response to insulin cellular response to insulin stimulus phosphatidylinositol-3-phosphate biosynthetic process glucose homeostasis response to peptide hormone positive regulation of GTPase activity positive regulation of phosphatidylinositol 3-kinase activity positive regulation of glycogen biosynthetic process positive regulation of glucose import negative regulation of insulin receptor signaling pathway positive regulation of insulin receptor signaling pathway negative regulation of insulin secretion phosphatidylinositol phosphorylation insulin-like growth factor receptor signaling pathway phosphatidylinositol-mediated signaling positive regulation of glucose import in response to insulin stimulus positive regulation of mesenchymal cell proliferation regulation of gene expression lipid catabolic process insulin secretion positive regulation of cell migration mammary gland development response to caffeine protein localization to nucleus positive regulation of phosphorylation protein kinase B signaling protein heterooligomerization positive regulation of glucagon secretion negative regulation of somatostatin secretion

Process(es)

signal transducer activity transmembrane receptor protein tyrosine kinase adaptor activity protein kinase C binding Ras guanyl-nucleotide exchange factor activity insulin receptor binding insulin-like growth factor receptor binding protein binding 1-phosphatidylinositol-3-kinase activity SH2 domain binding phosphatidylinositol 3-kinase binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity protein kinase binding protein domain specific binding