Bethyl Laboratories

IRS1 Polyclonal Antibody

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IRS1 Antibody in Immunohistochemistry (IHC) — **Detection of human IRS1 by immunohistochemistry.** *Sample:* FFPE section of human breast carcinoma. *Antibody:* Affinity purified rabbit anti-IRS1 (Cat. No. A301-158A Lot1) used at a dilution of 1:1,000 (1µg/ml). *Detection:* DAB.

IRS1 Antibody in Western Blot (WB) — **Detection of human and mouse IRS1 by western blot (h&m) and immunoprecipitation (h).** *Samples:* Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 µg) and mouse NIH3T3 (M; 50 µg) cells. *Antibodies:* Affinity purified rabbit anti-IRS1 antibody A301-158A used for WB at 0.04 µg/ml (A) and 1.0 µg/ml (B) and used for IP at 3 µg/mg lysate. IRS1 was also immunoprecipitated by rabbit anti-IRS1 antibody A301-157A, which recognizes an upstream epitope. *Detection:* Chemiluminescence with exposure times of 10 seconds (A and B).

Product Details

A301-158A

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:10,000

Immunohistochemistry (IHC)

1:1,000-1:5,000

Immunoprecipitation (IP)

2-5 µg/mg lysate

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Between 1192 and 1242

View immunogen

Conjugate

Unconjugated

Form

liquid

Concentration

0.20 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.0 to 8.0, with 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Target Information

IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Insulin receptor substrate 1; IRS-1

Gene Aliases: G972R; HIRS-1; IRS-1; IRS1

UniProt ID: (Human) P35568, (Mouse) P35569

Entrez Gene ID: (Human) 3667, (Mouse) 16367

Function(s)

Process(es)

signal transducer activity transmembrane receptor protein tyrosine kinase adaptor activity protein kinase C binding Ras guanyl-nucleotide exchange factor activity insulin receptor binding insulin-like growth factor receptor binding protein binding 1-phosphatidylinositol-3-kinase activity SH2 domain binding phosphatidylinositol 3-kinase binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity protein kinase binding protein domain specific binding