Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:2,000-1:10,000 | - |
Immunohistochemistry (IHC) |
1:1,000-1:5,000 | - |
Immunoprecipitation (IP) |
2-5 µg/mg lysate | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Between 1192 and 1242 |
Conjugate |
Unconjugated |
Form |
liquid |
Concentration |
0.20 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
TBS, pH 7.0 to 8.0, with 0.1% BSA |
Contains |
0.09% sodium azide |
Storage conditions |
4° C |
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Insulin receptor substrate 1; IRS-1
Gene Aliases: G972R; HIRS-1; IRS-1; IRS1
UniProt ID: (Human) P35568, (Mouse) P35569
Entrez Gene ID: (Human) 3667, (Mouse) 16367
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