Invitrogen

IRS1 Polyclonal Antibody

This Antibody was verified by IP Mass Spectrometry to ensure that the antibody binds to the antigen stated. View Details

1 Reference

View all (51) IRS1 antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

IRS1 Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of IRS1 was done on 70% confluent log phase T-47D cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with IRS1 Rabbit Polyclonal Antibody (Product # PA1-1059) at 1:250 dilution in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing Nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

IRS1 Antibody in IP-MS — IP-MS enrichment of IRS1 (LFQ intensity): IRS1 was enriched 44-fold from HCT116 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and IRS1 antibody (Product # PA1-1059). See more information on IP-MS verification of antibody selectivity. {IP-MS}

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1:250

Immunofluorescence (IF)

1:250

Western Blot (WB)

2 µg/mL

Published Applications

Western Blot (WB)

See 1 publication below

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues D(631) Y M P M S P K S V S A P Q Q I(646) of human IRS-1.

Conjugate

Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_325017

Product Specific Information

PA1-1059 detects IRS-1 in mouse and human cells.

PA1-1059 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~170 kDa protein representing IRS-1 from 3T3 L1 cell extract.

The PA1-1059 immunogen is a synthetic peptide corresponding to residues D(631) Y M P M S P K S V S A P Q Q I(646) of human IRS-1. The peptide sequence is 100% conservation in rats, and mice. This peptide (Cat. # PEP-193) is available for use in neutralization and control experiments.

Target Information

Insulin receptor substrates (IRS) are responsible for several insulin related activities, such as glucose homeostasis, cell growth, cell transformation, apoptosis and insulin signal transduction. Serine/threonine phosphorylation of IRS-1 has been demonstrated to be a negative regulator of insulin signaling and is responsible for its degradation, although IRS-1 degradation pathways are not well understood. IRS-1 has also been shown to be constitutively activated in cancers such as breast cancer, Wilm's tumors, and adrenal cortical carcinomas, thus making IRS-1 phosphorylation and subsequent degradation an attractive therapeutic option. To date there have been four subtypes identified: IRS-1,2,3, and 4, with IRS-1 being widely expressed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.