Product Specifications
Species Reactivity	Human, Mouse
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to residues D(631) Y M P M S P K S V S A P Q Q I(646) of human IRS-1. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 1mg/mL BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_325017

Product Specific Information

PA1-1059 detects IRS-1 in mouse and human cells.

PA1-1059 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~170 kDa protein representing IRS-1 from 3T3 L1 cell extract.

The PA1-1059 immunogen is a synthetic peptide corresponding to residues D(631) Y M P M S P K S V S A P Q Q I(646) of human IRS-1. The peptide sequence is 100% conservation in rats, and mice. This peptide (Cat. # PEP-193) is available for use in neutralization and control experiments.

Target Information

IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The hyperglycemia-induced inflammatory response in adipocytes: the role of reactive oxygen species.

The Journal of biological chemistry

Lin Y,Berg AH,Iyengar P,Lam TK,Giacca A,Combs TP,Rajala MW,Du X,Rollman B,Li W,Hawkins M,Barzilai N,Rhodes CJ,Fantus IG,Brownlee M,Scherer PE

PA1-1059 was used in western blot to study the important role for ROS production in adipocytes and the associated insulin resistance and inflammatory response