Proteintech

ISCU Polyclonal Antibody

Product Image Gallery

ISCU Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of MCF-7 cells, using ISCU antibody 14812-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

ISCU Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human endometrial cancer tissue slide using 14812-1-AP ( ISCU Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

ISCU Antibody in Western Blot (WB) — Mouse heart tissue were subjected to SDS PAGE followed by western blot with 14812-1-AP (ISCU antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

ISCU Antibody in Immunoprecipitation (IP) — IP result of anti-ISCU (IP:14812-1-AP, 3ug; Detection:14812-1-AP 1:500) with mouse heart tissue lysate 4000ug.

Product Details

14812-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 12 publications 12 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:20-1:200	View 1 publication 1 publication
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ISCU Fusion Protein Ag6562 (1-167 aa encoded by BC061903) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.29 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Two ISCU isoforms, ISCU1 (cytosolic, 15 kDa) and ISCU2 (mitochondrial, 18 kDa), were produced by alternative splicing. ISCU2 is considered the canonical isoform 1. Proteintech's ISCU antibody 14812-1-AP is raised against the full-length ISCU2 and is expected to detect 14, 15, and 18-19 kDa bands.

Immunogen sequence: MAAAGAFRL RRAASALLLR SPRLPARELS APARLYHKKV VDHYENPRNV GSLDKTSKNV GTGLVGAPAC GDVMKLQIQV DEKGKIVDAR FKTFGCGSAI ASSSLATEWV KGKTVEEALT IKNTDIAKEL CLPPVKLHCS MLAEDAIKAA LADYKLKQEP KKGEAEKK (1-167 aa encoded by BC061903)

Target Information

Iron-sulfur (Fe-S) clusters are necessary for several mitochondrial enzymes and other subcellular compartment proteins. They contain sulfur and iron, and are created via several steps that include cysteine desulfurases, iron donors, chaperones, and scaffold proteins. This gene encodes the two isomeric forms, ISCU1 and ISCU2, of the Fe-S cluster scaffold protein. Mutations in this gene have been found in patients with myopathy with severe exercise intolerance and myoglobinuria.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MicroRNA-210 Downregulates ISCU and Induces Mitochondrial Dysfunction and Neuronal Death in Neonatal Hypoxic-Ischemic Brain Injury.

Molecular neurobiology

Ma Q,Dasgupta C,Li Y,Huang L,Zhang L

Thu Aug 01 00:00:00 EDT 2019

GRP75 overexpression rescues frataxin deficiency and mitochondrial phenotypes in Friedreich ataxia cellular models.

Human molecular genetics

Dong YN,McMillan E,Clark EM,Lin H,Lynch DR

Wed May 15 00:00:00 EDT 2019

Phosphoinositide-3-kinase inhibition elevates ferritin level resulting depletion of labile iron pool and blocking of glioma cell proliferation.

Biochimica et biophysica acta. General subjects

Gupta P,Singh P,Pandey HS,Seth P,Mukhopadhyay CK

Fri Mar 01 00:00:00 EST 2019

The Favored Mechanism for Coping with Acute Cold Stress: Upregulation of miR-210 in Rats.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Guo W,Lian S,Zhen L,Zang S,Chen Y,Lang L,Xu B,Guo J,Ji H,Wang J,Fu S,Zhang L,Yang H

Wed Aug 01 00:00:00 EDT 2018

Dysfunction in the mitochondrial Fe-S assembly machinery leads to formation of the chemoresistant truncated VDAC1 isoform without HIF-1α activation.

PloS one

Ferecatu I,Canal F,Fabbri L,Mazure NM,Bouton C,Golinelli-Cohen MP

Mon Jul 09 00:00:00 EDT 2018

Dysfunction in the mitochondrial Fe-S assembly machinery leads to formation of the chemoresistant truncated VDAC1 isoform without HIF-1α activation.

PloS one

Ferecatu I,Canal F,Fabbri L,Mazure NM,Bouton C,Golinelli-Cohen MP

Mon Jul 09 00:00:00 EDT 2018

Iron accumulation in senescent cells is coupled with impaired ferritinophagy and inhibition of ferroptosis.

Redox biology

Masaldan S,Clatworthy SAS,Gamell C,Meggyesy PM,Rigopoulos AT,Haupt S,Haupt Y,Denoyer D,Adlard PA,Bush AI,Cater MA

Sun Apr 01 00:00:00 EDT 2018

Targeted Metabolomics Reveals Abnormal Hepatic Energy Metabolism by Depletion of β-Carotene Oxygenase 2 in Mice.

Scientific reports

Wu L,Guo X,Lyu Y,Clarke SL,Lucas EA,Smith BJ,Hildebrand D,Wang W,Medeiros DM,Shen X,Lin D

Tue Nov 07 00:00:00 EST 2017

Regulation of iron homeostasis by the p53-ISCU pathway.

Scientific reports

Funauchi Y,Tanikawa C,Yi Lo PH,Mori J,Daigo Y,Takano A,Miyagi Y,Okawa A,Nakamura Y,Matsuda K

Thu Nov 12 00:00:00 EST 2015

Regulation of iron homeostasis by the p53-ISCU pathway.

Scientific reports

Funauchi Y,Tanikawa C,Yi Lo PH,Mori J,Daigo Y,Takano A,Miyagi Y,Okawa A,Nakamura Y,Matsuda K

Thu Nov 12 00:00:00 EST 2015

The diabetes drug target MitoNEET governs a novel trafficking pathway to rebuild an Fe-S cluster into cytosolic aconitase/iron regulatory protein 1.

The Journal of biological chemistry

Ferecatu I,Gonçalves S,Golinelli-Cohen MP,Clémancey M,Martelli A,Riquier S,Guittet E,Latour JM,Puccio H,Drapier JC,Lescop E,Bouton C

Fri Oct 10 00:00:00 EDT 2014

Cochaperone binding to LYR motifs confers specificity of iron sulfur cluster delivery.

Cell metabolism

Maio N,Singh A,Uhrigshardt H,Saxena N,Tong WH,Rouault TA

Tue Mar 04 00:00:00 EST 2014

MicroRNA-210 decreases heme levels by targeting ferrochelatase in cardiomyocytes.

Journal of the American Heart Association

Qiao A,Khechaduri A,Kannan Mutharasan R,Wu R,Nagpal V,Ardehali H

Mon Apr 22 00:00:00 EDT 2013

MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity.

Science (New York, N.Y.)

Stehling O,Vashisht AA,Mascarenhas J,Jonsson ZO,Sharma T,Netz DJ,Pierik AJ,Wohlschlegel JA,Lill R

Fri Jul 13 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: Iron-sulfur cluster assembly enzyme ISCU, mitochondrial; iron-sulfur cluster scaffold homolog; IscU iron-sulfur cluster scaffold homolog; ISCU2; NifU-like N-terminal domain containing; NifU-like N-terminal domain-containing protein; NifU-like protein

Gene Aliases: 2310020H20Rik; AA407971; HML; hnifU; ISCU; ISU2; NIFU; NIFUN; RGD1309562

UniProt ID: (Human) Q9H1K1, (Mouse) Q9D7P6

Entrez Gene ID: (Human) 23479, (Mouse) 66383, (Rat) 288740

Molecular Function: chaperone