Proteintech

ISCU Polyclonal Antibody

Product Image Gallery

ISCU Antibody in Western Blot (WB) — Mouse heart tissue were subjected to SDS PAGE followed by western blot with 14812-1-AP (ISCU antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

ISCU Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human endometrial cancer tissue slide using 14812-1-AP ( ISCU Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

ISCU Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of MCF-7 cells, using ISCU antibody 14812-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

ISCU Antibody in Immunoprecipitation (IP) — IP result of anti-ISCU (IP:14812-1-AP, 3ug; Detection:14812-1-AP 1:500) with mouse heart tissue lysate 4000ug.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1000

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC)

1:20-1:200

Immunofluorescence (IF)

1:20-1:200

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ISCU Fusion Protein Ag6562

Conjugate

Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Two ISCU isoforms, ISCU1 (cytosolic, 15 kDa) and ISCU2 (mitochondrial, 18 kDa), were produced by alternative splicing. ISCU2 is considered the canonical isoform 1. Proteintech's ISCU antibody 14812-1-AP is raised against the full-length ISCU2 and is expected to detect 14, 15, and 18-19 kDa bands.

Target Information

Iron-sulfur (Fe-S) clusters are necessary for several mitochondrial enzymes and other subcellular compartment proteins. They contain sulfur and iron, and are created via several steps that include cysteine desulfurases, iron donors, chaperones, and scaffold proteins. This gene encodes the two isomeric forms, ISCU1 and ISCU2, of the Fe-S cluster scaffold protein. Mutations in this gene have been found in patients with myopathy with severe exercise intolerance and myoglobinuria.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Iron-sulfur cluster assembly enzyme ISCU, mitochondrial; iron-sulfur cluster scaffold homolog; IscU iron-sulfur cluster scaffold homolog; ISCU2; NifU-like N-terminal domain containing; NifU-like N-terminal domain-containing protein; NifU-like protein

Gene Aliases: 2310020H20Rik; AA407971; HML; hnifU; ISCU; ISU2; NIFU; NIFUN; RGD1309562

UniProt ID: (Human) Q9H1K1, (Mouse) Q9D7P6

Entrez Gene ID: (Human) 23479, (Mouse) 66383, (Rat) 288740

Function(s)

cellular iron ion homeostasis nitrogen fixation iron-sulfur cluster assembly small molecule metabolic process [2Fe-2S] cluster assembly protein maturation by iron-sulfur cluster transfer

Process(es)

iron ion binding protein binding ferrous iron binding protein complex scaffold iron-sulfur transferase activity 2 iron, 2 sulfur cluster binding 4 iron, 4 sulfur cluster binding metal ion binding iron-sulfur cluster binding