Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:1,000 | View 12 publications 12 publications |
Immunohistochemistry (IHC) |
- | View 1 publication 1 publication |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:50-1:500 | - |
Immunocytochemistry (ICC/IF) |
1:20-1:200 | View 1 publication 1 publication |
Immunoprecipitation (IP) |
0.5-4.0 µg | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Rat |
Published species |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
ISCU Fusion Protein Ag6562 (1-167 aa encoded by BC061903) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.29 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C |
Two ISCU isoforms, ISCU1 (cytosolic, 15 kDa) and ISCU2 (mitochondrial, 18 kDa), were produced by alternative splicing. ISCU2 is considered the canonical isoform 1. Proteintech's ISCU antibody 14812-1-AP is raised against the full-length ISCU2 and is expected to detect 14, 15, and 18-19 kDa bands.
Immunogen sequence: MAAAGAFRL RRAASALLLR SPRLPARELS APARLYHKKV VDHYENPRNV GSLDKTSKNV GTGLVGAPAC GDVMKLQIQV DEKGKIVDAR FKTFGCGSAI ASSSLATEWV KGKTVEEALT IKNTDIAKEL CLPPVKLHCS MLAEDAIKAA LADYKLKQEP KKGEAEKK (1-167 aa encoded by BC061903)
Iron-sulfur (Fe-S) clusters are necessary for several mitochondrial enzymes and other subcellular compartment proteins. They contain sulfur and iron, and are created via several steps that include cysteine desulfurases, iron donors, chaperones, and scaffold proteins. This gene encodes the two isomeric forms, ISCU1 and ISCU2, of the Fe-S cluster scaffold protein. Mutations in this gene have been found in patients with myopathy with severe exercise intolerance and myoglobinuria.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Iron-sulfur cluster assembly enzyme ISCU, mitochondrial; iron-sulfur cluster scaffold homolog; IscU iron-sulfur cluster scaffold homolog; ISCU2; NifU-like N-terminal domain containing; NifU-like N-terminal domain-containing protein; NifU-like protein
Gene Aliases: 2310020H20Rik; AA407971; HML; hnifU; ISCU; ISU2; NIFU; NIFUN; RGD1309562
UniProt ID: (Human) Q9H1K1, (Mouse) Q9D7P6
Entrez Gene ID: (Human) 23479, (Mouse) 66383, (Rat) 288740
Molecular Function:
chaperone
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