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Invitrogen
ISG15 Polyclonal Antibody
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FIGURE: 1 / 5
ISG15 Antibody (PA5-17461) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1), A549 treated with IFNa (1000U/mL for 24 hours) (Lane 2), HeLa (Lane 3), HeLa treated with IFNa (1000U/mL for 24 hours) (Lane 4) and MCF7 (Lane 5). The blot was blocked with 2% BSA for 1h, probed with Anti-ISG15 Polyclonal Antibody (Product # PA5-17461, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 17kDa band... View More
Product Details
PA5-17461
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to amino acids from human ISG15 protein
Conjugate
Form
Liquid
Concentration
34 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with other ubiquitin family members, including ubiquitin, SUMO1, SUMO2, SUMO3 and NEDD8.
Target Information
Ubiquitin-like protein ISG15 plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. It is secreted from monocytes in response to type I interferons and causes natural killer (NK)-cell proliferation and an augmentation of non-MCH (major histocompatibility complex)-restricted cytotoxicity. Synthesis is stimulated by IFN-alpha or IFN-beta or IFN-omega, but not IFN-gamma. ISG15 expression is also induced by overexpression of interferon regulatory factors that participate in transcriptional regulation of IFN genes, and by influenza B virus. ISG15 is secreted by cell lines of monocyte, T-lymphocyte, B-lymphocyte, human fibroblasts, and epithelial origins. Enhancement of NK cell proliferation, augmentation of non-major histocompatibility complex-restricted cytotoxicity, and induction of IFN-gamma from T cells identify ISG15 as a member of the cytokine cascade and suggest that it may be responsible for amplifying and directing some of the immunomodulatory effects of IFN-alpha or IFN-beta. ISG15 has has also been shown to function intracellularly as a ubiquitin homolog. Mutations affecting the gene can result in Immunodeficiency 38, with basal ganglia calcification (IMD38).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: hUCRP; interferon, alpha-inducible protein; interferon, alpha-inducible protein (clone IFI-15K); Interferon-induced 15 kDa protein; interferon-induced 15-KDa protein; Interferon-induced 17 kDa protein; interferon-induced 17-kDa/15-kDa protein; interferon-stimulated protein (15 kDa); interferon-stimulated protein 15; interferon-stimulated protein, 15 kDa; IP17; Ubiquitin cross-reactive protein; Ubiquitin-like protein ISG15
Gene Aliases: G1P2; hUCRP; IFI15; IGI15; IMD38; IP17; Irfp; ISG15; UCRP
UniProt ID: (Human) Q5SVA4, (Mouse) Q64339
Entrez Gene ID: (Human) 9636, (Mouse) 100038882
modification-dependent protein catabolic process
translesion synthesis
negative regulation of protein ubiquitination
ISG15-protein conjugation
negative regulation of type I interferon production
regulation of interferon-gamma production
response to type I interferon
defense response to bacterium
negative regulation of viral genome replication
positive regulation of erythrocyte differentiation
defense response to virus
type I interferon signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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