Immunofluorescence analysis of CD49b/ITGA2 was performed using 70% confluent log phase U-2 OS cells. The cells were fixed with 4% paraformaldehyde for 10 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CD49b/ITGA2 Sheep Polyclonal Antibody (Product # PA5-47193) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Sheep IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A-11015) at a dilution of 1:2000 for 45 minutes at r... View More

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ITGA2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines and mouse tissues owing to their inherent genetic constitution. Relative expression of CD49b was observed among MCF7, MDA-MB-231, T-47D, A-431, U-2 OS, HEK-293, A549, U-937, Mouse Spleen, Mouse Thymus and Mouse Heart tissues using CD49b Polyclonal Antibody (Product # PA5-47193) in western blot where U-937 is reported to be negative for CD49b expression. (U937 being null: DOI: 10.1371/journal.pone.0008743) {RE}

Immunogen image

Immunogen Image ITGA2 Polyclonal Antibody

Product Details

PA5-47193

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

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Immunocytochemistry (ICC/IF)

5-15 µg/mL

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Flow Cytometry (Flow)

0.25 µg per million cells

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Sheep / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha 2/CD49b Tyr27-Thr1129

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2607349

Product Specific Information

In direct ELISAs and Western blots, approximately10% cross-reactivity with recombinant human Integrin alpha M is observed and less than 1% cross-reactivity with recombinant mouse (rm) Integrin alpha 5 and rmIntegrin alpha E is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

CD49b (Integrin alpha 2, Integrin alpha 2 chain, VLA-2 alpha chain, HM alpha 2) is a member of the integrin family. It is a glycoprotein with molecular weight of 150 kD, and it complexes with CD29 (Integrin beta 1) to form the heterodimeric integrin VLA-2 (integrin alpha 2 beta 1, or GPIa/IIa) complex. VLA-2 is an extracellular receptor for laminin, collagen, and fibronectin, and interaction with its ligands results in the activation of intracellular signaling pathways. It has reported roles in VEGF-induced angiogenesis in vivo, as well as adhesion and lymphocyte activation. CD49b is expressed by NK cells, NK-T cells, monocytes, platelets, and epithelial cells. It is also expressed on adaptive immune cells such as T and B cells, specifically on a subset of CD4+ T cells in the spleen, on intraepithelial and lamina propria lymphocytes in the intestine, as well as on a population of peripheral CD4+ type 1 T regulatory (Tr1) cells that co-express LAG-3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: alpha 2 integrin; alpha 2 subunit of VLA-2 receptor; BDPLT9; CD49 antigen-like family member B; CD49b; Collagen receptor; glycoprotein Ia; GPIa; human platelet alloantigen 5; human platelet alloantigen system 5; Integrin alpha-2; integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor); platelet antigen Br; platelet glycoprotein GPIa; Platelet membrane glycoprotein Ia; unnamed protein product; very late activation protein 2 receptor, alpha-2 subunit; very late activation receptor alpha-2 subunit; very late activation receptor subunit alpha-2; VLA-2 receptor, alpha 2 subunit; VLA-2 subunit alpha; VLA2 receptor

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Gene Aliases: BR; CD49B; DX5; GPIa; HPA-5; ITGA2; VLA-2; VLAA2

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UniProt ID: (Human) P17301, (Mouse) Q62469

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Entrez Gene ID: (Human) 3673, (Mouse) 16398

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Function(s)

beta-amyloid binding virus receptor activity integrin binding protein binding collagen binding signaling receptor activity collagen receptor activity laminin binding heparan sulfate proteoglycan binding macromolecular complex binding metal ion binding collagen binding involved in cell-matrix adhesion protein complex binding protein heterodimerization activity integrin

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ITGA2 Polyclonal Antibody

ITGA2 Antibody (PA5-47193) in ICC/IF

Product Details

PA5-47193

Western Blot (WB)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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