Invitrogen

ITGA2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (63) CD49b (Integrin alpha 2) antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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ITGA2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of CD49b/ITGA2 was performed using 70% confluent log phase U-2 OS cells. The cells were fixed with 4% paraformaldehyde for 10 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CD49b/ITGA2 Sheep Polyclonal Antibody (Product # PA5-47193) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Sheep IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A-11015) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing membrane localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

ITGA2 Antibody in Western Blot (WB) — Western blot was performed using Anti-ITGA2 Polyclonal Antibody (Product # PA5-47193) and a 160kDa band corresponding to ITGA2 was observed across cell lines and tissues tested except U-937. Whole cell extracts (30 µg lysate) of MCF7 (Lane 1), MDA-MB-231 (Lane 2), T-47D (Lane 3), A-431 (Lane 4), U-2 OS (Lane 5), HEK-293 (Lane 6), A549 (Lane 7), U-937 (Lane 8), Mouse Spleen (Lane 9), Mouse Thymus (Lane 10) and Mouse Heart (Lane 11) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.1 µg/mL) and detected by chemiluminescence Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Note: An uncharacterized band at (*) ~110kDa was observed in Mouse Heart tissue.

ITGA2 Antibody in Flow Cytometry (Flow) — Flow cytometry of ITGA2 in Mouse splenocytes were stained with Mouse Anti-Mouse CD161/NK1.1 PE‚conjugated Monoclonal Antibody. Samples were incubated in either (A) ITGA2 polyclonal antibody (Product # PA5-47193) or (B) Normal Sheep IgG Control followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody.

ITGA2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines and mouse tissues owing to their inherent genetic constitution. Relative expression of CD49b was observed among MCF7, MDA-MB-231, T-47D, A-431, U-2 OS, HEK-293, A549, U-937, Mouse Spleen, Mouse Thymus and Mouse Heart tissues using CD49b Polyclonal Antibody (Product # PA5-47193) in western blot where U-937 is reported to be negative for CD49b expression. (U937 being null: DOI: 10.1371/journal.pone.0008743)

Product Details

PA5-47193

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1 µg/mL

Immunocytochemistry (ICC/IF)

5-15 µg/mL

Flow Cytometry (Flow)

0.25 µg per million cells

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Sheep / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha 2/CD49b Tyr27-Thr1129

View immunogen

Conjugate

Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2607349

Product Specific Information

In direct ELISAs and Western blots, approximately10% cross-reactivity with recombinant human Integrin alpha M is observed and less than 1% cross-reactivity with recombinant mouse (rm) Integrin alpha 5 and rmIntegrin alpha E is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

CD49b (Integrin alpha 2, Integrin alpha 2 chain, VLA-2 alpha chain, HM alpha 2) is a member of the integrin family. It is a glycoprotein with molecular weight of 150 kD, and it complexes with CD29 (Integrin beta 1) to form the heterodimeric integrin VLA-2 (integrin alpha 2 beta 1, or GPIa/IIa) complex. VLA-2 is an extracellular receptor for laminin, collagen, and fibronectin, and interaction with its ligands results in the activation of intracellular signaling pathways. It has reported roles in VEGF-induced angiogenesis in vivo, as well as adhesion and lymphocyte activation. CD49b is expressed by NK cells, NK-T cells, monocytes, platelets, and epithelial cells. It is also expressed on adaptive immune cells such as T and B cells, specifically on a subset of CD4+ T cells in the spleen, on intraepithelial and lamina propria lymphocytes in the intestine, as well as on a population of peripheral CD4+ type 1 T regulatory (Tr1) cells that co-express LAG-3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: alpha 2 subunit of VLA-2 receptor; BDPLT9; CD49 antigen-like family member B; CD49b; Collagen receptor; GPIa; human platelet alloantigen system 5; Integrin alpha-2; integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor); platelet antigen Br; platelet glycoprotein GPIa; Platelet membrane glycoprotein Ia; very late activation protein 2 receptor, alpha-2 subunit; VLA-2 receptor, alpha 2 subunit; VLA-2 subunit alpha; VLA2 receptor

Gene Aliases: BR; CD49B; DX5; GPIa; HPA-5; ITGA2; VLA-2; VLAA2

UniProt ID: (Human) P17301, (Mouse) Q62469

Entrez Gene ID: (Human) 3673, (Mouse) 16398

Molecular Function: integrin