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Invitrogen
ITK Polyclonal Antibody
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FIGURE: 1 / 1
ITK Antibody (PA5-29668) in WB
Western Blot using ITK Polyclonal Antibody (Product # PA5-29668). Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ITK Polyclonal Antibody (Product # PA5-29668) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Product Details
PA5-29668
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human ITK. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: Jurkat.
Predicted reactivity: Mouse (95%), Rat (95%), Rhesus Monkey (99%), Bovine (97%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ITK is a tyrosine kinase expressed in T-cells which contains both SH2 and SH3 domains. ITK is thought to play a role in T-cell proliferation and differentiation. The Tec family of non-receptor tyrosine kinases is composed of six proteins designated Tec, Itk (also known as Emt or Tsk), Btk (previously known as Atk, BPK or Emb), Bmx, Txk (also known as Rlk) and Dsrc28C. All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain pleckstrin homology (PH) and Tec homology (TH) domains in their amino termini. Itk associates with CD28 and becomes activated subsequent to CD28 ligation.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: homolog of mouse T-cell itk/tsk; IL-2-inducible T-cell kinase; Interleukin-2-inducible T-cell kinase; Kinase EMT; MGC126257; MGC126258; T-cell-specific kinase; Tyrosine-protein kinase ITK/TSK; Tyrosine-protein kinase Lyk; unnamed protein product
Gene Aliases: EMT; ITK; LPFS1; LYK; PSCTK2
UniProt ID: (Human) Q08881
Entrez Gene ID: (Human) 3702
positive regulation of cytokine production
NK T cell differentiation
adaptive immune response
immune system process
cellular defense response
signal transduction
intracellular signal transduction
T cell activation
gamma-delta T cell activation
T cell receptor signaling pathway
B cell receptor signaling pathway
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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