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Invitrogen
ITK Polyclonal Antibody
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FIGURE: 1 / 5
ITK Antibody (PA5-29669) in ICC/IF
Immunofluorescence analysis of Tyrosine-protein kinase ITK/TSK was performed using Jurkat and Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with ITK Polyclonal Antibody (Product # PA5-29669) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A3... View More
Product Details
PA5-29669
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human ITK. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.31 mg/mL
Amount
31 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: Molt-4, Jurkat.
Predicted reactivity: Mouse (92%), Rat (93%), Rhesus Monkey (98%), Bovine (94%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ITK is a tyrosine kinase expressed in T-cells which contains both SH2 and SH3 domains. ITK is thought to play a role in T-cell proliferation and differentiation. The Tec family of non-receptor tyrosine kinases is composed of six proteins designated Tec, Itk (also known as Emt or Tsk), Btk (previously known as Atk, BPK or Emb), Bmx, Txk (also known as Rlk) and Dsrc28C. All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain pleckstrin homology (PH) and Tec homology (TH) domains in their amino termini. Itk associates with CD28 and becomes activated subsequent to CD28 ligation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: homolog of mouse T-cell itk/tsk; IL-2-inducible T-cell kinase; interleukin-2-inducible T cell kinase; Interleukin-2-inducible T-cell kinase; Kinase EMT; Kinase TLK; MGC126257; MGC126258; T-cell-specific kinase; Tyrosine-protein kinase ITK/TSK; Tyrosine-protein kinase Lyk; unnamed protein product
Gene Aliases: EMT; ITK; LPFS1; LYK; PSCTK2; Tcsk; Tlk; Tsk
UniProt ID: (Human) Q08881, (Mouse) Q03526
Entrez Gene ID: (Human) 3702, (Mouse) 16428
nucleotide binding
protein kinase activity
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
protein binding
ATP binding
zinc ion binding
kinase activity
transferase activity
metal ion binding
transferase activity, transferring phosphorus-containing groups
GTPase-activating protein
positive regulation of cytokine production
NK T cell differentiation
adaptive immune response
immune system process
cellular defense response
signal transduction
intracellular signal transduction
T cell activation
gamma-delta T cell activation
T cell receptor signaling pathway
B cell receptor signaling pathway
cytokine production
protein phosphorylation
transmembrane receptor protein tyrosine kinase signaling pathway
activation of phospholipase C activity
phosphorylation
peptidyl-tyrosine phosphorylation
interferon-gamma production
interleukin-4 production
peptidyl-tyrosine autophosphorylation
regulation of cell proliferation
innate immune response
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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