Western blot analysis of ITM2B in (lane 2) Gene specific transient over-expression HEK293T cell lysate and (lane 3) Empty vector transfected control HEK293T cell lysate. Samples were probed with an ITM2B polyclonal antibody (Product # PA1-25724) at a dilution of 1:1000. Secondary detection was performed using a peroxidase conjugated Rabbit anti-Chicken IgY (IgG) at a 1:10,000 dilution and developed with TMB Substrate.
|Tested species reactivity||Rat, Human, Mouse|
|Host / Isotype||Chicken / IgY|
|Immunogen||Synthetic peptide corresponding to residues 1-60 of ITM2B.|
|Purification||Antigen affinity chromatography|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:2,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-25724 detects ITM2B from human, mouse, and rat samples.
PA1-25724 has been successfully used in Western blot procedures.
The PA1-25724 immunogen is a synthetic peptide corresponding to residues 1-60 of ITM2B.
Amyloid precursor proteins are processed by beta-secretase and gamma-secretase to produce beta-amyloid peptides which form the characteristic plaques of Alzheimer disease. This gene encodes a transmembrane protein which is processed at the C-terminus by furin or furin-like proteases to produce a small secreted peptide which inhibits the deposition of beta-amyloid. Mutations which result in extension of the C-terminal end of the encoded protein, thereby increasing the size of the secreted peptide, are associated with two neurogenerative diseases, familial British dementia and familial Danish dementia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.