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FIGURE: 1 / 1
ITPKB Antibody (PA5-120876) in WB
Western blot analysis of ITPKB in extracts of various cell lines. Samples were incubated with ITPKB Polyclonal antibody (Product # PA5-120876) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-120876
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ITPKB (NP_002212.3).
Conjugate
Form
Liquid
Concentration
1.11 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914448
Product Specific Information
Positive test controls include: HeLa, HepG2, BT-474, Mouse lung.
Immunogen sequence: MAVYCYALNS LVIMNSANEM KSGGGPGPSG SETPPPPRRA VLSPGSVFSP GRGASFLFPP AESLSPEEPR SPGGWRSGRR RLNSSSGSGS GSSGSSVSSP SWAGRLRGDR QQVVAAGTLS PPGPEEAKRK LRILQRELQN VQVNQKVGMF EAHIQAQSSA IQAPRSPRLG RARSPSPCPF RSSSQPPGRV LVQGARSEER RTKSWGEQCP ETSGTDSGRK GGPSLCSSQV KKGMPPLPGR AAPTGSEAQG PSAFVRMEKG
Target Information
Inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) regulates the level of calcium within the cell by releasing calcium from intracellular stores. Ins(1,4,5)P3 is phosphorylated by inositol 1,4,5-trisphosphate 3-kinase (IP3K) to form inositol 1,3,4,5-tetrakisphosphate (Ins(1,4,5)P4), which is is thought to regulate the influx of calcium across the plasma membrane. IP3K exists as three isoforms, IP3KA, B, and C. IP3KA, the most highly characterized isoform, is expressed in rat brain and testis. IP3KB is expressed in various rat tissues such as lung, thymus, testis, brain, and heart. IP3K activity is stimulated in the presence of calmodulin via phosphorylation by cAMP-dependent protein kinase, protein kinase C, or calcium/calmodulin dependent protein kinase II and, subsequently, mediates the inositol phosphate signaling pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Inositol 1,4,5-trisphosphate 3-kinase B; Inositol-trisphosphate 3-kinase B; insP 3-kinase B; IP3 3-kinase B; IP3K B; IP3K-B; proliferation-inducing protein 37
Gene Aliases: 1110033J02Rik; E130307H12Rik; IP3-3KB; IP3K; IP3K-B; IP3KB; ITPKB; PIG37
UniProt ID: (Human) P27987, (Rat) P42335
Entrez Gene ID: (Human) 3707, (Mouse) 320404, (Rat) 54260
MAPK cascade
regulation of protein phosphorylation
myeloid cell homeostasis
signal transduction
cell surface receptor signaling pathway
inositol trisphosphate metabolic process
negative regulation of neutrophil apoptotic process
common myeloid progenitor cell proliferation
inositol phosphate metabolic process
positive thymic T cell selection
negative regulation of myeloid cell differentiation
positive regulation of Ras protein signal transduction
positive regulation of alpha-beta T cell differentiation
cellular response to calcium ion
phosphorylation
T cell differentiation
thymic T cell selection
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