|Tested species reactivity||Human, Mouse|
|Published species reactivity||Rat, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 147 of Human Iba1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Contains||0.025% ProClin 300|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-27436 targets Iba1 in FACS, ICC/IF, IHC-P and WB applications and shows reactivity with Human and mouse samples. For IHC-P, heat mediated antigen retrieval using 10mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0) is suggested.
The PA5-27436 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 147 of Human Iba1.
This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Analysis of the behavioral, cellular and molecular characteristics of pain in severe rodent spinal cord injury.
PA5-27436 was used in western blot to investigate mechanisms associated with chronic pain after SCI
|Lee-Kubli CA,Ingves M,Henry KW,Shiao R,Collyer E,Tuszynski MH,Campana WM||Experimental neurology (278:91)||2016|
The Transient Receptor Potential Melastatin 2 (TRPM2) Channel Contributes to ß-Amyloid Oligomer-Related Neurotoxicity and Memory Impairment.
PA5-27436 was used in immunohistochemistry - free floating to investigate the contribution of TRPM2 to beta-amyloid neuronal toxicity
|Ostapchenko VG,Chen M,Guzman MS,Xie YF,Lavine N,Fan J,Beraldo FH,Martyn AC,Belrose JC,Mori Y,MacDonald JF,Prado VF,Prado MA,Jackson MF||The Journal of neuroscience : the official journal of the Society for Neuroscience (35:15157)||2015|