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Icam-1 Polyclonal Antibody

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Icam-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse liver tissue slide using 10020-1-AP (Icam-1 antibody) at dilution of 1:200 (under 40x lens).

Icam-1 Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 10020-1-AP (Icam-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Product Details

10020-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:4,000	View 15 publications 15 publications
Immunohistochemistry (IHC)	-	View 8 publications 8 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunohistochemistry (PFA fixed) (IHC (PFA))	1:200	-
Immunocytochemistry (ICC/IF)	-	View 3 publications 3 publications
Flow Cytometry (Flow)	Assay-dependent	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse, Rat
Published species	Hamster, Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein
Conjugate	Unconjugated
Form	Liquid
Concentration	1.23 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody is raised against mouse Icam-1 which is a cell surface sialoglycoprotein with an apparent molecular weight of 95-100 kDa.

Target Information

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Effects of Qingshen Granules on the Oxidative Stress-NF/kB Signal Pathway in Unilateral Ureteral Obstruction Rats.

Evidence-based complementary and alternative medicine : eCAM

Jin H,Wang Y,Wang D,Zhang L

Thu Oct 01 00:00:00 EDT 2020

Control release of mitochondria-targeted antioxidant by injectable self-assembling peptide hydrogel ameliorated persistent mitochondrial dysfunction and inflammation after acute kidney injury.

Drug delivery

Zhao M,Zhou Y,Liu S,Li L,Chen Y,Cheng J,Lu Y,Liu J

Thu Nov 01 00:00:00 EDT 2018

Mechanosensing by β1 integrin induces angiocrine signals for liver growth and survival.

Nature

Lorenz L,Axnick J,Buschmann T,Henning C,Urner S,Fang S,Nurmi H,Eichhorst N,Holtmeier R,Bódis K,Hwang JH,Müssig K,Eberhard D,Stypmann J,Kuss O,Roden M,Alitalo K,Häussinger D,Lammert E

Mon Oct 01 00:00:00 EDT 2018

Mechanosensing by β1 integrin induces angiocrine signals for liver growth and survival.

Nature

Lorenz L,Axnick J,Buschmann T,Henning C,Urner S,Fang S,Nurmi H,Eichhorst N,Holtmeier R,Bódis K,Hwang JH,Müssig K,Eberhard D,Stypmann J,Kuss O,Roden M,Alitalo K,Häussinger D,Lammert E

Mon Oct 01 00:00:00 EDT 2018

Expression and significance of Sirt1 in renal allografts at the early stage of chronic renal allograft dysfunction.

Transplant immunology

Xia Y,Deng J,Zhou Q,Shao X,Yang X,Sha M,Zou H

Fri Jun 01 00:00:00 EDT 2018

Extracts of Zuo Jin Wan, a traditional Chinese medicine, phenocopies 5-HTR1D antagonist in attenuating Wnt/β-catenin signaling in colorectal cancer cells.

BMC complementary and alternative medicine

Pan J,Xu Y,Song H,Zhou X,Yao Z,Ji G

Tue Nov 28 00:00:00 EST 2017

Mechanical Activation of Hypoxia-Inducible Factor 1α Drives Endothelial Dysfunction at Atheroprone Sites.

Arteriosclerosis, thrombosis, and vascular biology

Feng S,Bowden N,Fragiadaki M,Souilhol C,Hsiao S,Mahmoud M,Allen S,Pirri D,Ayllon BT,Akhtar S,Thompson AAR,Jo H,Weber C,Ridger V,Schober A,Evans PC

Wed Nov 01 00:00:00 EDT 2017

BTP2, a Store-Operated Calcium Channel Inhibitor, Attenuates Lung Ischemia-Reperfusion Injury in Rats.

Inflammation

Zhang W,Qi Z,Wang Y

Thu Jun 01 00:00:00 EDT 2017

Heparanase-driven inflammation from the AGEs-stimulated macrophages changes the functions of glomerular endothelial cells.

Diabetes research and clinical practice

Xu G,Qin Q,Yang M,Qiao Z,Gu Y,Niu J

Wed Feb 01 00:00:00 EST 2017

In vitro assessment of deferoxamine on mesenchymal stromal cells from tumor and bone marrow.

Environmental toxicology and pharmacology

Wang G,Shen G,Yin T

Sun Jan 01 00:00:00 EST 2017

Glucocorticoid-Induced Leucine Zipper Suppresses ICAM-1 and MCP-1 Expression by Dephosphorylation of NF-κB p65 in Retinal Endothelial Cells.

Investigative ophthalmology & visual science

Gu R,Lei B,Jiang C,Xu G

Sun Jan 01 00:00:00 EST 2017

Puerarin Improves Diabetic Aorta Injury by Inhibiting NADPH Oxidase-Derived Oxidative Stress in STZ-Induced Diabetic Rats.

Journal of diabetes research

Li W,Zhao W,Wu Q,Lu Y,Shi J,Chen X

Tue Dec 20 00:00:00 EST 2016

Neuroprotective effects of Kaempferide-7-O-(4″-O-acetylrhamnosyl)-3-O-rutinoside on cerebral ischemia-reperfusion injury in rats.

European journal of pharmacology

Wang S,Xu H,Xin Y,Li M,Fu W,Wang Y,Lu Z,Yu X,Sui D

Wed Oct 05 00:00:00 EDT 2016

Human β-defensin-3 alleviates the progression of atherosclerosis accelerated by Porphyromonas gingivalis lipopolysaccharide.

International immunopharmacology

Li L,Bian T,Lyu J,Cui D,Lei L,Yan F

Thu Sep 01 00:00:00 EDT 2016

Photoreceptor Cells Influence Retinal Vascular Degeneration in Mouse Models of Retinal Degeneration and Diabetes.

Investigative ophthalmology & visual science

Liu H,Tang J,Du Y,Saadane A,Tonade D,Samuels I,Veenstra A,Palczewski K,Kern TS

Mon Aug 01 00:00:00 EDT 2016

FTY720 Attenuates Retinal Inflammation and Protects Blood-Retinal Barrier in Diabetic Rats.

Investigative ophthalmology & visual science

Fan L,Yan H

Tue Mar 01 00:00:00 EST 2016

FTY720 Attenuates Retinal Inflammation and Protects Blood-Retinal Barrier in Diabetic Rats.

Investigative ophthalmology & visual science

Fan L,Yan H

Tue Mar 01 00:00:00 EST 2016

Anti-inflammation Effects of Oxysophoridine on Cerebral Ischemia-Reperfusion Injury in Mice.

Inflammation

Wang YS,Li YX,Zhao P,Wang HB,Zhou R,Hao YJ,Wang J,Wang SJ,Du J,Ma L,Sun T,Yu JQ

Tue Dec 01 00:00:00 EST 2015

Early treatment with atorvastatin exerts parenchymal and vascular protective effects in experimental cerebral ischaemia.

British journal of pharmacology

Potey C,Ouk T,Petrault O,Petrault M,Berezowski V,Salleron J,Bordet R,Gautier S

Sun Nov 01 00:00:00 EDT 2015

Endothelial precursor cells promote angiogenesis in hepatocellular carcinoma.

World journal of gastroenterology

Sun XT,Yuan XW,Zhu HT,Deng ZM,Yu DC,Zhou X,Ding YT

Fri Sep 21 00:00:00 EDT 2012

Bone marrow cells: Important role on neovascularization of hepatocellular carcinoma.

Journal of gastroenterology and hepatology

Zhu H,Shao Q,Sun X,Deng Z,Yuan X,Zhou X,Ding Y

Sun Jul 01 00:00:00 EDT 2012

Effects of pegylated interferon α2b on metastasis of hepatocellular carcinoma.

The Journal of surgical research

Arakawa Y,Shimada M,Utsunomiya T,Imura S,Morine Y,Ikemoto T

Sun Jan 01 00:00:00 EST 2012

Toll-like receptor 4 signaling by follicular dendritic cells is pivotal for germinal center onset and affinity maturation.

Immunity

Garin A,Meyer-Hermann M,Contie M,Figge MT,Buatois V,Gunzer M,Toellner KM,Elson G,Kosco-Vilbois MH

Fri Jul 23 00:00:00 EDT 2010

Tumor-derived intercellular adhesion molecule-1 mediates tumor-associated leukocyte infiltration in orthotopic pancreatic xenografts.

Experimental biology and medicine (Maywood, N.J.)

Roland CL,Dineen SP,Toombs JE,Carbon JG,Smith CW,Brekken RA,Barnett CC

Mon Feb 01 00:00:00 EST 2010

Tumor-derived intercellular adhesion molecule-1 mediates tumor-associated leukocyte infiltration in orthotopic pancreatic xenografts.

Experimental biology and medicine (Maywood, N.J.)

Roland CL,Dineen SP,Toombs JE,Carbon JG,Smith CW,Brekken RA,Barnett CC

Mon Feb 01 00:00:00 EST 2010

Cyclosporin A and phenytoin modulate inflammatory responses.

Journal of dental research

Suzuki AM,Yoshimura A,Ozaki Y,Kaneko T,Hara Y

Tue Dec 01 00:00:00 EST 2009

Effects of sodium beta-aescin on expression of adhesion molecules and migration of neutrophils after middle cerebral artery occlusion in rats.

Acta pharmacologica Sinica

Hu XM,Zhang Y,Zeng FD

Thu Jul 01 00:00:00 EDT 2004

Effects of sodium beta-aescin on expression of adhesion molecules and migration of neutrophils after middle cerebral artery occlusion in rats.

Acta pharmacologica Sinica

Hu XM,Zhang Y,Zeng FD

Thu Jul 01 00:00:00 EDT 2004

Bioinformatics

Protein Aliases: CD54; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; Ly 47; MALA-2; MALA2; MyD10; sCD54; soluble CD54; Surface antigen of activated B cells

Gene Aliases: CD54; ICAM; Icam-1; Icam1; Ly-47; MALA-2

UniProt ID: (Mouse) P13597, (Rat) Q00238

Entrez Gene ID: (Mouse) 15894, (Rat) 25464

Function(s)

Process(es)

integrin binding protein complex binding

Icam-1 Polyclonal Antibody

Product Details

10020-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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