|Tested species reactivity||Bovine, Dog, Human, Mouse, Rat|
|Host / Isotype||Goat / IgG|
|Immunogen||synthetic peptide SSFNFGGPAPGMN, corresponding to C terminal amino acids 1026-1038 of Human Importin 7.|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.1-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-31257 detects Importin 7 from human samples.
PA1-31257 has been successfully used in Western blot applications.
The PA1-31257 immunogen is: Synthetic peptide: SSFNFGGPAPGMN, corresponding to C terminal amino acids 1026-1038 of Human Importin 7.
The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-beta. Similar to importin-beta, this protein prevents the activation of RaNULLs GTPase by RanGAP1 and inhibits nucleotide exchange on RanGTP, and also binds directly to nuclear pore complexes where it competes for binding sites with importin-beta and transportin. This protein has a Ran-dependent transport cycle and it can cross the nuclear envelope rapidly and in both directions. At least four importin beta-like transport receptors, namely importin beta itself, transportin, RanBP5 and RanBP7, directly bind and import ribosomal proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.