Immunofluorescent analysis of Influenza A M2 in A/WSN/33 infected Vero cells. A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min and stained using an Influenza A M2 polyclonal antibody (Product # PA5-32233) diluted at 1:500. Blue: DAPI staining. Yellow: WGA life stained at 37°C, 30 min.
|Tested species reactivity||Virus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment of influenza A virus M2 (A/Puerto Rico/8/1934(H1N1))|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-32233 targets Influenza A Virus M2 in Western blot, immunocytochemistry, and immunofluorescence applications and shows reactivity with Virus samples.
The PA5-32233 immunogen is recombinant protein fragment of influenza A virus M2 (A/Puerto Rico/8/1934(H1N1)).
Influenza A virus matrix 1, M1, is a critical protein required for assembly and budding. Hemagglutinin associates with M1 via its cytoplasmic tail and transmembrane domain. The M2 and nucleoprotein proteins are critical in the replication cycle of influenza viruses. The M2 channel protein is an essential component of the viral envelope because of its ability to form a highly selective, pH-regulated, proton-conducting channel. The M2 channel allows protons to enter the interior of the virus and acidification weakens the interaction of the M1 protein with the ribonuclear core.
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