Immunofluorescent analysis of Influenza A NP in A/WSN/33 infected Vero cells. A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min and stained using an Influenza A NP polyclonal antibody (Product # PA5-32242) diluted at 1:500. Blue: DAPI staining. Yellow: WGA life stained at 37°C, 30 min.
|Tested species reactivity||Virus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment of Influenza A virus NP (A/WSN/1933(H1N1))|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-32242 targets Influenza A virus NP in Western blot, immunocytochemistry, and immunofluorescence applications and shows reactivity with Virus samples.
The PA5-32242 immunogen is recombinant protein fragment of Influenza A virus NP (A/WSN/1933(H1N1)).
Influenza A viruses are single stranded, negative sense RNA viruses. Influenza A nucleoprotein (NP) associates with its RNA genome and is present in eight separate segments of ribonucleoprotein (RNP), each of which has to be present for successful replication.
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