Proteintech

Insulin Polyclonal Antibody

View all (28) Insulin antibodies

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Insulin Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of ( 4% PFA) fixed human pancreas tissue using 15848-1-AP (Insulin antibody) at dilution of 1:500 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Insulin Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of (4% PFA) fixed human pancreas tissue using 15848-1-AP (Insulin antibody) at dilution of 1:500 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Insulin Antibody in Immunofluorescence (IF) — IF result of Insulin antibody (15848-1-AP, 1:500) with Rat pancreas.

Insulin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas using 15848-1-AP (INS antibody) at dilution of 1:1000 (under 10x lens).

Insulin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas using 15848-1-AP (INS antibody) at dilution of 1:1000 (under 40x lens).

Insulin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas using 15848-1-AP (INS antibody) at dilution of 1:500 (under 10x lens).

Insulin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas using 15848-1-AP (INS antibody) at dilution of 1:500 (under 40x lens).

Insulin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse pancreas using 15848-1-AP (INS antibody) at dilution of 1:50 (under 10x lens).

Insulin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse pancreas using 15848-1-AP (INS antibody) at dilution of 1:50 (under 40x lens).

Insulin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded rat pancreas using 15848-1-AP (INS antibody) at dilution of 1:50 (under 40x lens).

Insulin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded rat pancreas using 15848-1-AP (INS antibody) at dilution of 1:50 (under 10x lens).

Insulin Antibody in Western Blot (WB) — Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15848-1-AP (Insulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:250-1:1000

-

Immunofluorescence (IF)

1:250-1:1000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:1000

-

Western Blot (WB)

1:500-1:2000

View 6 publications 6 publications

Immunohistochemistry (IHC)

-

View 5 publications 5 publications

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Insulin Fusion Protein Ag8630

Conjugate

Unconjugated

Form

Liquid

Concentration

0.28 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Target Information

Insulin is a peptide hormone secreted by beta cells of the pancreatic islets. It regulates carbohydrate, protein and lipid metabolism by enhancing membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Insulin is 51-amino acid polypeptide product produced from a precursor peptide, proinsulin. Proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds and one molecule of C-peptide. Insulin deficiency results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of b-cell origin such as insulinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Insulin; Insulin A chain; Insulin B chain; Insulin-1; Insulin-1 A chain; Insulin-1 B chain; Insulin-2; Insulin-2 A chain; Insulin-2 B chain; preproinsulin; proinsulin

Gene Aliases: AA986540; IDDM; IDDM1; IDDM2; ILPR; INS; Ins-1; Ins-2; Ins1; Ins2; Ins2-rs1; InsII; IRDN; Mody; MODY10; Mody4; proinsulin

UniProt ID: (Human) P01308, (Rat) P01322, (Mouse) P01325, (Rat) P01323, (Mouse) P01326

Entrez Gene ID: (Human) 3630, (Rat) 24505, (Mouse) 16333, (Rat) 24506, (Mouse) 16334

negative regulation of transcription from RNA polymerase II promoter activation of MAPK activity glucose metabolic process ER overload response myoblast fusion biological_process insulin receptor signaling pathway lipid catabolic process regulation of protein binding positive regulation of phosphatidylinositol 3-kinase activity positive regulation of peptidyl-tyrosine phosphorylation canonical Wnt signaling pathway intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress MAPK cascade negative regulation of acute inflammatory response regulation of cellular amino acid metabolic process acute-phase response G-protein coupled receptor signaling pathway positive regulation of cell proliferation response to organic substance positive regulation of phosphatidylinositol 3-kinase signaling glucose transport regulation of transmembrane transporter activity activation of protein kinase B activity positive regulation of cellular protein metabolic process negative regulation of protein oligomerization regulation of protein localization negative regulation of NAD(P)H oxidase activity response to cytokine wound healing negative regulation of protein catabolic process glucose homeostasis positive regulation of MAPK cascade response to peptide hormone positive regulation of glycogen biosynthetic process positive regulation of DNA replication negative regulation of glycogen catabolic process positive regulation of glycolytic process positive regulation of mitotic nuclear division negative regulation of proteolysis negative regulation of fatty acid metabolic process positive regulation of glucose import positive regulation of insulin receptor signaling pathway alpha-beta T cell activation regulation of protein secretion negative regulation of protein secretion positive regulation of cytokine secretion negative regulation of lipid catabolic process positive regulation of NF-kappaB transcription factor activity response to cAMP positive regulation of protein kinase B signaling fatty acid homeostasis negative regulation of respiratory burst involved in inflammatory response positive regulation of respiratory burst positive regulation of protein localization to nucleus negative regulation of feeding behavior regulation of transcription, DNA-templated ER to Golgi vesicle-mediated transport cell-cell signaling positive regulation of gene expression positive regulation of cell growth positive regulation of cell migration positive regulation of protein autophosphorylation cellular protein metabolic process positive regulation of nitric oxide biosynthetic process positive regulation of cell differentiation negative regulation of gluconeogenesis negative regulation of vasodilation positive regulation of vasodilation positive regulation of lipid biosynthetic process positive regulation of nitric-oxide synthase activity positive regulation of peptide hormone secretion positive regulation of brown fat cell differentiation negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway carbohydrate metabolic process activation of JUN kinase activity regulation of gene expression myotube differentiation regulation of phosphorylation

Process(es)

protease binding insulin receptor binding insulin-like growth factor receptor binding hormone activity protein binding identical protein binding chaperone binding protein complex binding