Invitrogen

Insulin/Proinsulin Antibody Cocktail

8 References

View all (3) Insulin/Proinsulin antibodies

Datasheet

Protocols

Tech Support

Datasheet

Protocols

Tech Support

Antibody Testing Data (1)

Insulin/Proinsulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 1

Insulin/Proinsulin Antibody (MA5-12042) in IHC (P)

Formalin-fixed, paraffin-embedded human pancreas stained with Insulin antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of beta cells of the islets of Langerhans.

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Product Details

MA5-12042

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1.0 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Product Specifications

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Cocktail

Type

Antibody

Clone

INS04, INS05

Immunogen

Insulin protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10985260

Product Specific Information

MA5-12042 targets Insulin (INS04 + INS05) in IHC (P) applications and shows reactivity with Human, Porcine, and Rat samples.

The MA5-12042 immunogen is insulin protein.

Target Information

Insulin is a peptide hormone secreted by beta cells of the pancreatic islets. It regulates carbohydrate, protein and lipid metabolism by enhancing membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Insulin is 51-amino acid polypeptide product produced from a precursor peptide, proinsulin. Proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds and one molecule of C-peptide. Insulin deficiency results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of b-cell origin such as insulinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Accelerated Generation of Extra-Islet Insulin-Producing Cells in Diabetic Rats, Treated with Sodium Phthalhydrazide.

International journal of molecular sciences

Abidov MT,Sokolova KV,Gette IF,Danilova IG

MA5-12042 was used in Immunohistochemistry (Paraffin) to find that APH modulates macrophage production and increases the proliferative activity of pancreatic β-cells.

Wed Apr 13 00:00:00 EDT 2022

Enteroendocrine cells are a potential source of serum autotaxin in men.

Biochimica et biophysica acta

Bolier R,Tolenaars D,Kremer AE,Saris J,Parés A,Verheij J,Bosma PJ,Beuers U,Oude Elferink RPJ

MA5-12042 was used in immunohistochemistry - paraffin section to discover a potential source of serum autotaxin in men by analyzing enteroendocrine cells

Fri Apr 01 00:00:00 EDT 2016

The potential role of combined antioxidant treatment on pancreas of STZ-diabetic mice.

Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie

Karatug A,Bolkent S

MA5-12042 was used in immunohistochemistry to study mitochondrial and cytosolic oxidative stress in streptozotocin-diabetic mouse pancreas and the effect of combined antioxidant therapy

Fri Mar 01 00:00:00 EST 2013

Transient in vitro epigenetic reprogramming of skin fibroblasts into multipotent cells.

Biomaterials

Zhu XQ,Pan XH,Wang W,Chen Q,Pang RQ,Cai XM,Hoffman AR,Hu JF

MA5-12042 was used in immunocytochemistry to study the transient in vitro epigenetic reprogramming of skin fibroblasts into multipotent cells

Thu Apr 01 00:00:00 EDT 2010

Antidiabetic effects of dipeptidyl peptidase-IV inhibitors and sulfonylureas in streptozotocin-nicotinamide-induced mildly diabetic mice.

Metabolism: clinical and experimental

Matsuyama-Yokono A,Tahara A,Nakano R,Someya Y,Shiraki K,Hayakawa M,Shibasaki M

MA5-12042 was used in immunohistochemistry to study the antidiabetic effect of DPP-IV inhibitors and sulfoylureas in mildly diabetic mice

Sun Mar 01 00:00:00 EST 2009

Immunohistochemical expression of rabphilin-3A-like (Noc2) in normal and tumor tissues of human endocrine pancreas.

Biotechnic & histochemistry : official publication of the Biological Stain Commission

Shanmugam C,Katkoori VR,Jhala NC,Grizzle WE,Manne U

MA5-12042 was used in immunohistochemistry to study Noc2 expression in normal and tumor tissues of the human endocrine pancreas

Wed Apr 01 00:00:00 EDT 2009

Antioxidant and pancreas-protective effect of aucubin on rats with streptozotocin-induced diabetes.

European journal of pharmacology

Jin L,Xue HY,Jin LJ,Li SY,Xu YP

MA5-12042 was used in immunohistochemistry to study the antioxidant and pancreas-protective effect of aucubin in streptozotocin-induced diabetes

Mon Mar 17 00:00:00 EDT 2008

Extracellular ATP and zinc are co-secreted with insulin and activate multiple P2X purinergic receptor channels expressed by islet beta-cells to potentiate insulin secretion.

Purinergic signalling

Richards-Williams C,Contreras JL,Berecek KH,Schwiebert EM

MA5-12042 was used in immunohistochemistry to examine the role of extracellular ATP and zinc in insulin secretion

Mon Dec 01 00:00:00 EST 2008

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