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Invitrogen
ITGB5 Polyclonal Antibody
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FIGURE: 1 / 6
ITGB5 Antibody (PA5-17260) in ICC/IF
Immunofluorescence analysis of ITGB5 was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ITGB5 Polyclonal Antibody (Product # PA5-17260) at 1:25 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034... View More
Product Details
PA5-17260
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues surrounding Lys789 of human integrin β5 protein
Conjugate
Form
Liquid
Concentration
60 µg/mL
Amount
6 µg
Purification
Antigen affinity chromatography, Protein A
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
Integrins are important extracellular matrix (ECM) receptor proteins located on cell surfaces. They are hetrodimers composed of an alpha and a beta transmembrane glycoprotein subunit. Around 22 different integrins (different alpha/ beta subunit combinations) are found in nature. Integrins are generally present in high concentrations at the cell surface, but, unlike most other cell-surface receptors, they bind ligands with very low affinity. Due to their weak individual binding, integrins need to cluster and bind in-groups in order to effectively bind the ECM. Integrins bind many different ligands including laminin. Each integrin is made up of a large N-terminal extracellular domain that binds the ECM ligand and a small C-terminal cytoplasmic domain that mediates interaction with the actin cytoskeleton and signaling function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD61; FLJ26658; GP3A; GPIIIa; Integrin beta-5; integrin beta-5 subunit; integrin beta-5 subunit precursor; testis secretory sperm-binding protein Li 217p; unnamed protein product
Gene Aliases: ITGB5
UniProt ID: (Human) P18084
Entrez Gene ID: (Human) 3693
formation of primary germ layer
cell adhesion
cell-matrix adhesion
transforming growth factor beta receptor signaling pathway
integrin-mediated signaling pathway
cell migration
cell adhesion mediated by integrin
wound healing, spreading of epidermal cells
endodermal cell differentiation
stress fiber assembly
viral entry into host cell
epithelial cell-cell adhesion
cell-cell adhesion
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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